FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424598
Sequences flagged as poor quality0
Sequence length20-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT15450.36387359337538094No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11100.2614237466968756No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9870.23245516936019484No Hit
TATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGAAGCTCTGGCTCTCCAAGG7660.1804059369097358No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGTTAGTGTAAGAAATTGCCATTTGAAA7520.17710870046491034No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA7430.1749890484646654No Hit
ATGTTTATGCATATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAA7280.17145629513092384No Hit
CCTGAAAGAGGTATGCTCTCTTAGGAATACAGTTTCTAGCATTAAACAAA7060.16627492357476956No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6430.15143735957305499No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT6170.14531392046123628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.13636427868242432No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC5790.13636427868242432No Hit
ATGTAAAACCAACTCCCTGCCACCAAAATAATTAAAATAGTCACATTGTT5550.1307118733484378No Hit
GCCTTGGAGAGCCAGAGCTTCCGCATTTTCTTTACTATTCTTTTTAAAAA5520.13000532268168952No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT5300.1248239511255352No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG4770.1123415560129817No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG4450.10480501556766636No Hit
CTGCTATCCTGACTAATTTAAGTCATTGCTGACTGCATAGCTCTTTTTCT4300.1012722622339248No Hit
GTAATAAATATGCTAACTTTCTGAATCAAAATGGAGAGCCTCTCAAGAAA4300.1012722622339248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCA556.031152E-661.63060870
CCGTCGT200.006550353553.28941747
CCTAACG253.3732018E-452.2229232
CTAACGC253.3732018E-452.2229233
GTCGTTT200.007660488651.22113839
TGTCGTA409.1947913E-750.94494638
ATGTCGT652.6193447E-1046.76819237
GCCGTCT2500.045.2301246
TATGCCG3400.045.12486643
AATGTCG752.1827873E-1144.86107336
ATGCCGT3200.044.8567844
ACGCGTA4300.044.27018427
CGCGTAG4400.044.1039928
GGTATCA6250.043.8672521
GCGTAGT4400.043.4389529
GTAATGT851.8189894E-1243.2267634
CGAGACG4400.043.06006223
AGACGCG4450.042.65668525
CGTATGC3750.042.4312341
GACGCGT4550.041.768526
Produced by FastQC (version 0.11.5)