FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424598
Sequences flagged as poor quality0
Sequence length20-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40860.9623220081112016No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11610.27343510803159693No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9580.22562517958162778No Hit
ATGTTTATGCATATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAA7160.16863009246393057No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGTTAGTGTAAGAAATTGCCATTTGAAA7090.16698147424151785No Hit
TATATGTTCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGAAGCTCTGGCTCTCCAAGG6780.15968045068511863No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT6550.15426356224004822No Hit
CCTGAAAGAGGTATGCTCTCTTAGGAATACAGTTTCTAGCATTAAACAAA6540.15402804535113213No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA6080.14319426846099134No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT5700.13424462668217937No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC5670.13353807601543108No Hit
ATGTAAAACCAACTCCCTGCCACCAAAATAATTAAAATAGTCACATTGTT5390.12694360312578015No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT5270.1241174004587869No Hit
GCCTTGGAGAGCCAGAGCTTCCGCATTTTCTTTACTATTCTTTTTAAAAA4980.11728741068021988No Hit
CTGCTATCCTGACTAATTTAAGTCATTGCTGACTGCATAGCTCTTTTTCT4820.11351914045756221No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG4710.11092845467948506No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT4500.10598260001224687No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG4410.10386294801200194No Hit
TAATTGACCTATGTTTATGCATATATGTTCTCTACACAGTGAAACTTTTT4310.10150777912284091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG402.564775E-1065.135362
TGGTATC1250.059.9245382
CTAACGC457.3123374E-1057.8844683
GACTATC200.00488711857.38330559
GCACCGC200.005591430755.46438653
AAGCGAC200.007607016751.31193539
GTGGTAT1550.046.2468951
GGTATCA6450.042.9388851
CGTTGCA350.001790874337.2070613
TATCAAC20000.034.684582
GATCGAA909.027644E-932.5868822
ACCTAAC601.982451E-532.583041
TCAACGC21700.032.106094
AATAGTC2100.032.08664736
ATCAACG21850.031.889443
AAGCGGT1951.2727469E-831.44040370
CAACGCA22500.031.1092365
TAGTCAC2100.030.7559538
AACGCAG23250.030.2457396
GTATCAA24250.030.0973221
Produced by FastQC (version 0.11.5)