FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641384
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30510.47569007022314247No Hit
CTATAAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGAGTGTGAGAGTGAATTCTGTC28000.4365559477629626No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTGCAGATACA17250.26894964638968233No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15090.2352724732765395No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14130.22030484078180934No Hit
GCTTTATAGTTTCCCGTACACTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCAT13060.2036221670637247No Hit
GTGTGAGAGTGAATTCTGTCCCAGACCCACTGCCGCTGAACCTTGATGGGACCCCACTTTGCAAGGTGGATGCAG12410.1934878325620845No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12230.1906814014693226No Hit
GCTCTATGCTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCA11390.17758472303643372No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11280.17586968181307921No Hit
GTGTATTACTGTGCGAGAGGTTACGATTTTTGGGGCTACGGTGTGGACGT11200.17462237910518505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10870.16947725543512154No Hit
GCATAGAGCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC10820.1686976912426877No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTTACCATCACTT10680.16651491150387288No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10390.1619934391877565No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10160.1584074439025607No Hit
ATGCTGCATCCACCTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCACTCTCA9610.14983223778578825No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9480.1478053708854602No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9140.14250433437690993No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9030.14078929315355543No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9000.14032155463809512No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8980.14000972896112157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8950.13954199044566126No Hit
GAGTTACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAACCAGGGAAAG8560.13346138974467714No Hit
CTCTCACACTCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTTATAGTTTCCCGTACA8360.13034313297494168No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT8330.12987539445948137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8200.12784852755915332No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8120.12660122485125916No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC7920.1234829680815237No Hit
CTTTTGGCCAGGGGACCAGGCTGGAGATCAAACGAACTGTGGCTGCACCA7830.12207975253514275No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAATAGTTAC7740.1206765369887618No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7700.12005288563481471No Hit
GAGCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAAT7610.11864967008843376No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGAGTGTGAGAGTGAATTCTGTCCCAGACCCACTGCCG7240.11288089506442317No Hit
GTAGGAGACAGAGTTACCATCACTTGCCGGGCCAGTCAGGGCATTAGCAGTTATTTAGCCTGGTATCAGCAAAAA7200.11225724371047609No Hit
GTCTGGGACAGAATTCACTCTCACACTCAGCAGCCTGCAGCCTGAAGATT7070.11023037681014806No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6930.10804759707133324No Hit
GTATCAACGCAGAGTACGGGAAACTATAAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG6850.10680029436343906No Hit
ATGCAGCATAGAGCAGGAGCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCTAAATAACTGCTAATGCC6810.10617664300949198No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6600.10290247340126975No Hit
GGCCAAAAGTGTACGGGAAACTATAAAGCTGTTGACAGTAATAAGTTGCA6560.10227882204732267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6550.10212290920883589No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6540.10196699637034912No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6500.10134334501640202No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6500.10134334501640202No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6490.10118743217791526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA200.002493657468.0093769
GACCCTA350.003634058261.8385170
GGTACGT250.00982618948.09572268
GTGGTAT2500.043.121941
AATGGTA2950.041.5750470
TCCGAGA1701.9099389E-938.1943770
GTATCAA20400.035.340441
TCTGGAG6400.034.94519870
GACAGGT601.5789194E-533.689021
GCTCGGG2800.033.49585770
TCTCCGA2300.033.1093768
ATCTCCG2100.032.4905967
TATCAAC22450.031.6532972
CTCCGAG2650.030.79669469
ATCAACG23550.030.1653883
TCAACGC23650.030.040184
AACGCAG24050.029.6805576
CAACGCA24150.029.418235
TCATGCC500.005792901429.28109263
CGCAGAG24550.028.8017628
Produced by FastQC (version 0.11.5)