FastQCFastQC Report
Wed 8 May 2024
EGAF00004692293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534502
Total Bases38.5 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT34300.6417188336058611No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10060.18821257918585899No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10010.18727712899109827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.18166442782253386No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG9230.17268410595283085No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7900.14780113077219542No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACGTCAGAATCAGGGAGA7450.13938207901934885No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7260.1358273682792581No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6890.12890503683802867No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG6880.12871794679907653No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6830.1277824966043158No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6810.1274083165264115No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC6570.12291815559156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG6290.1176796345008999No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT6170.11543455403347416No Hit
GTATATTTCTGTCAGCAGTCTGATACTTCAGTATTCACTTTCGGCCCTGG6100.11412492376080911No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT6070.11356365364395268No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG5980.11187984329338338No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5870.10982185286490975No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC5630.10533169193005826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT5570.10420915169634538No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT5560.10402206165739325No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT5530.10346079154053679No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC5480.10252534134577607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.10009317083939817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)