FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534502
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80231.501023382513068No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8550.15996198330408493No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG7610.1423755196425832No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6680.1249761460200336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6450.12067307512413424No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGACGTCAGAATCAGGGAGA6420.1201118050072778No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC6120.11449910383871341No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6080.11375074368290483No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6020.11262820344919197No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA5570.10420915169634538No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA5480.10252534134577607No Hit
GTATATTTCTGTCAGCAGTCTGATACTTCAGTATTCACTTTCGGCCCTGGGACCAGAGTGGATCTCAAACGAA5480.10252534134577607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG500.065.969122
CTAACGC551.8189894E-1259.9606973
TACGTAT502.0343291E-457.8427670
AATTCGA250.005277579756.28006469
GCGCGAC200.00877003549.518
TCCGAGA2250.038.5618470
TGTACCC1001.6370905E-1136.26943212
ATGTACC1052.7284841E-1134.54878211
TTCGATC807.22448E-834.4214739
ACGGGTA1350.034.2190216
GTACGTA550.002743173234.1091369
CGGAAAT1005.2568794E-1033.4701330
GTATCAA22700.033.2969861
TGCAACG502.3273886E-433.2083527
GGTACGT500.003168797433.1209668
ACGGCAC502.3978036E-433.04022223
AACGCCA1006.166374E-1032.9814725
TCGATCC851.18694516E-732.53012540
GGTATCA10050.032.513551
TTTCGGG951.3511453E-831.26901419
Produced by FastQC (version 0.11.5)