FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840925
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29410.34973392395279007No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18360.2183310045485626No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC17650.2098879210393317No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16690.19847192080149836No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14720.17504533698011118No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14250.1694562535303386No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14030.16684008680916848No Hit
GCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTCCCGAAGACATAAGAGGTCCTGCTGCCTGCAAATGAG13520.16077533668281951No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC13500.16053750334453132No Hit
GATTATTACTGCAGCTCATTTGCAGGCAGCAGGACCTCTTATGTCTTCGG12960.15411600321075006No Hit
GTCCTGGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12520.14888366976840978No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG12170.14472158634836638No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11990.14258108630377264No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG11990.14258108630377264No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA11980.14246216963462854No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATTT11460.13627850283913548No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC11390.1354460861551268No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACCGGAACCAGCAGTGACGTT11100.13199750274994798No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG10770.12807325266819275No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10130.12046258584297054No Hit
GTCGATCACCATCTCCTGCACCGGAACCAGCAGTGACGTTGGTGGTTACAATTATGTGTCCTGGTACCAACAATA9920.11796533579094451No Hit
CTCCTGCACCGGAACCAGCAGTGACGTTGGTGGTTACAATTATGTGTCCTGGTACCAACAATACCCAGGCAAAGT9540.1134465023634688No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9400.11178166899545143No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9270.11023575229657817No Hit
ACCCAGGACGGTGACCTTGGTCCCAGTCCCGAAGACATAAGAGGTCCTGC9220.10964116895085768No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT9140.1086898355977049No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG8920.10607366887653477No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCCAGGACGGTGACCTTGGTCCCAGTCCCGAAGACATAAGAGGTC8880.10559800219995838No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8730.10381425216279692No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8680.10321966881707642No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG8630.10262508547135595No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8590.10214941879477955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCC200.00789902550.830123
TGGTATC2600.040.4058342
GGTATCA8100.040.190761
TTAGTAG450.001277887639.85546569
GTGGTAT2700.038.93481
GTATCAA21400.037.08021
GACAGGT1400.033.9109541
TATCAAC24150.032.8363152
ATCAACG24550.032.161353
TCAACGC24500.032.0886734
CAACGCA25000.031.4506425
AACGCAG25600.030.978296
GTACTAT450.005028140730.1430681
GTAGACC450.005045728830.1215533
CGCAGAG27400.028.943228
CAGAGTA27500.028.71473310
AGAGTAC27700.028.38505611
GCAGAGT28300.028.1425199
ATAGTAC907.93505E-626.356363
CCTACGT808.5085725E-526.32156851
Produced by FastQC (version 0.11.5)