FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436921
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58550.4074684690390077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC56650.3942457518541382No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44410.3090636158842414No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44310.30836768340082715No Hit
TTATAATACTTATTCTCCGAAGTACACTTTTGGCCAGGGGACCAACCTGG43680.30398330875531776No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC43200.30064283283492965No Hit
GTATTATAATGTTGGCAGTAATAAGCTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC40500.28185265578274654No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40260.2801824178225525No Hit
GAGTATCAGTACCTGGTTGGCCTGGTATCAGCTGAAACCAGGGAGAGCCCCTAAACTCCTGATCTTTAAGGCGT39940.27795543387562716No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA36890.25672949313149435No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35200.24496823416179456No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAGCTTA34870.24267165696652773No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31540.21949710526883523No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31340.21810524030200684No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG30770.21413842514654596No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATCAGTACCTGGTTGGCCTGGTATCAGCTGAAA30720.2137904589048389No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30500.21225940744132765No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29650.20634398133230705No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29630.2062047948356242No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29390.20453455687543015No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29180.20307309866026035No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA28110.19562662108772855No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27110.18866729625358666No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26700.18581397307158848No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26230.1825430903995418No Hit
GCTTATTACTGCCAACATTATAATACTTATTCTCCGAAGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAA24800.1725912558867189No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT24620.17133857741657335No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24120.1678589149995024No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23960.1667454230260397No Hit
CTGATACTCTGACTGGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA23090.16069081042033626No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC23070.1605516239236534No Hit
GTAATAAGCTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG23050.1604124374269706No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG22590.1572111480032653No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22540.15686318176155822No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA22460.15630643577482686No Hit
GGCCAAAAGTGTACTTCGGAGAATAAGTATTATAATGTTGGCAGTAATAAGCTGCAAAATCATCAGGCTGCAGG21990.15303555310278016No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21840.15199165437765888No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21420.14906873794731929No Hit
GTACCTGGTTGGCCTGGTATCAGCTGAAACCAGGGAGAGCCCCTAAACTC21420.14906873794731929No Hit
GTTTAGGGGCTCTCCCTGGTTTCAGCTGATACCAGGCCAACCAGGTACTG21400.14892955145063647No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC21240.14781605947717374No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT20810.14482354979849277No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20560.14308371858995728No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20530.14287493884493302No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20340.14155266712644604No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20050.1395344629245449No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19680.1369595127359124No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19100.13292310433211013No Hit
ACCAGGTACTGATACTCTGACTGGCCCGACAAGTGATGGTGACTCTGTCT18810.13090490013020895No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA18550.12909547567333207No Hit
ACCTTGATGGGACCCCACTTACTAAACTAGACGCCTTAAAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTCAG18380.12791239045152797No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18010.12533744026289545No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17840.12415435504109133No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17740.12345842255767714No Hit
CCCCTAAACTCCTGATCTTTAAGGCGTCTAGTTTAGTAAGTGGGGTCCCA17620.12262330357758011No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17510.12185777784582451No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17040.11858689517377782No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATCAGTAC16990.11823892893207072No Hit
CCTTAAAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTCAGCTGATACCAGGCCAACCAGGTACTGATACTCTGA16480.11468967326665835No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16320.11357618129319566No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16310.11350658804485424No Hit
TCTTTAAGGCGTCTAGTTTAGTAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT16260.11315862180314715No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15940.11093163785622173No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15890.11058367161451464No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15840.11023570537280755No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15820.1100965188761247No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG15720.10940058639271052No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAGCTTATTACTGCCAACATTATAATACTTATTCTCCGAAGTA15710.1093309931443691No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15440.10745197543915079No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15360.10689522945241944No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15210.10585133072729816No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15050.10473783875383545No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14980.10425068601544552No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14910.10376353327705559No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14890.10362434678037274No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14810.1030676007936414No Hit
GTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTG14740.10258044805525146No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14630.10181492232349586No Hit
TACTTATTCTCCGAAGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCAT14550.10125817633676451No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14380.10007509111496038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT252.630041E-454.93120239
CTAACGC409.923533E-750.3976483
CGTATGC5500.047.6830541
TCGTATG5600.046.7196940
ATCTCGT5350.046.63327437
AATCTCG5500.045.8740536
CCCACGA8000.045.36577619
ATGCCGT5450.045.2930244
GTATGCC6050.045.16426542
GGCAATC6550.045.06445733
ACGAGAC8000.044.95511222
CGAGACA8300.043.74431623
TATACAC8500.043.0850453
CCTAACG551.8091305E-742.7646142
CTTATAC8500.042.701661
GCAATCT6550.042.55679334
CACGAGA8450.042.55660221
AGGCAAT8050.042.5140732
GCCCACG8600.042.19631618
CAAGAGG8750.041.2065328
Produced by FastQC (version 0.11.5)