FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436921
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61490.42792888405138485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57340.399047685989696No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46830.3259051819828647No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45720.3181803314169672No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44610.3104554808510697No Hit
TTATAATACTTATTCTCCGAAGTACACTTTTGGCCAGGGGACCAACCTGG41220.2868633696633287No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC38890.27064814279977817No Hit
GAGTATCAGTACCTGGTTGGCCTGGTATCAGCTGAAACCAGGGAGAGCCCCTAAACTCCTGATCTTTAAGGCGT38250.2661941749059273No Hit
GTATTATAATGTTGGCAGTAATAAGCTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC38060.26487190318744036No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35080.24413311518169753No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34640.2410710122546751No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33900.2359211118774101No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAGCTTA33350.23209348321863205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31990.22262880144419908No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30370.21135469521288922No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30120.20961486400435375No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27430.19089428020051205No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27340.19026794096543928No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATCAGTACCTGGTTGGCCTGGTATCAGCTGAAA27050.18824973676353815No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA26940.18748421103178253No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGA26890.18713624479007543No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26450.18407414186305301No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26440.1840045486147116No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26210.18240390390285896No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCG26120.1817775646677862No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25380.17662766429052118No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23980.16688460952272255No Hit
GTAATAAGCTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG23780.16549274455589416No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23660.16465762557579713No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23640.1645184390791143No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT23360.16256982812555457No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23120.1608995901653605No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCCCCTGGCCAAAAGTG22670.15776789398999666No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22610.15735033449994815No Hit
GCTTATTACTGCCAACATTATAATACTTATTCTCCGAAGTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAA22440.15616724927814402No Hit
CTGATACTCTGACTGGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGA22030.15331392609614586No Hit
GTTTAGGGGCTCTCCCTGGTTTCAGCTGATACCAGGCCAACCAGGTACTG21580.150182229920782No Hit
CTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTGCACCA21500.14962548393405067No Hit
GGCCAAAAGTGTACTTCGGAGAATAAGTATTATAATGTTGGCAGTAATAAGCTGCAAAATCATCAGGCTGCAGG21330.14844239871224651No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG21190.14746809323546667No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21040.14642419451034538No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19890.1384209709510822No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19860.13821219120605793No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19800.13779463171600942No Hit
GTACCTGGTTGGCCTGGTATCAGCTGAAACCAGGGAGAGCCCCTAAACTC19340.13459334229230419No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19280.13417578280225567No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18520.12888669592830782No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGGTTGGTCC18520.12888669592830782No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC18310.12742523771313802No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG17840.12415435504109133No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17810.12394557529606708No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCAGAGTATCAGTAC17290.12032672638231329No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17230.11990916689226477No Hit
ACCAGGTACTGATACTCTGACTGGCCCGACAAGTGATGGTGACTCTGTCT17070.11879567491880208No Hit
CCCCTAAACTCCTGATCTTTAAGGCGTCTAGTTTAGTAAGTGGGGTCCCA16840.11719503020694944No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16830.11712543695860803No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16720.11635991122685241No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16710.116290317978511No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16610.1155943854950968No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16520.11496804626002403No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC16370.11392414753490275No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG16290.1133674015481714No Hit
ACCTTGATGGGACCCCACTTACTAAACTAGACGCCTTAAAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTCAG16150.11239309607139153No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16090.11197553658134302No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15910.11072285811119748No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG15900.11065326486285607No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15760.10967895938607619No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15540.10814790792256498No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15420.10731278894246796No Hit
GTACACTTTTGGCCAGGGGACCAACCTGGAGATCAAACGAACTGTGGCTG15370.10696482270076085No Hit
TCTTTAAGGCGTCTAGTTTAGTAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15190.10571214423061531No Hit
CCTTAAAGATCAGGAGTTTAGGGGCTCTCCCTGGTTTCAGCTGATACCAGGCCAACCAGGTACTGATACTCTGA15080.10494661849885971No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14980.10425068601544552No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14750.10265004130359288No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14680.10216288856520295No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14500.1009102100950574No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAGCTTATTACTGCCAACATTATAATACTTATTCTCCGAAGTA14410.10028387085998464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCGA200.007773859451.0377435
ACGGTAT450.00973875548.20592570
CCTAACG405.9019774E-541.9332922
GACGGTA500.001890586636.8037669
GTATCAA46800.036.4316181
CTAACGC502.1929826E-433.55133
TATCAAC52900.031.7710042
ATCAACG52800.031.7085233
TCAACGC53300.031.4066944
AACGCAG54050.031.1570956
ATGGCAA6050.031.07489470
CAACGCA54550.030.810015
TGTTAGA21000.029.95653770
GTGCTAG3950.029.7393551
CGCAGAG56950.029.3348948
ACGCAGA57550.029.0873477
AGAGTAC58000.028.86167111
GCAGAGT58700.028.5174949
GTGGTAT6250.027.9244061
TACGTAT1306.5814523E-427.8111170
Produced by FastQC (version 0.11.5)