FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455823
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA72870.5005416180401051No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57570.3954464244623144No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCT47030.3230475133309475No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41060.2820397809349076No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40370.27730019377355625No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36440.2503051538545551No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34450.2366359097225418No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCTCCGTGGACGTTCGGCCA34390.23622377170851128No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT31040.21321273259180545No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC30920.21238845656374436No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30280.20799231774741847No Hit
CATCTATGGTGCATCCGGCAGGGCCACTGGCATCCCAGACAGGTTCAGAGGCAGTGGGTCTGGGACAGACTTCA29690.20393962727611806No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29680.20387093760711297No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC29180.20043645415685837No Hit
GTATGGTAGCTCACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27600.18958348645405382No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCT27280.18738541704589087No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26690.18333272657459046No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26070.17907396709627477No Hit
GTCCCAGACCCACTGCCTCTGAACCTGTCTGGGATGCCAGTGGCCCTGCC25520.1752960353009947No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG25510.1752273456319896No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24590.16890789608352114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24450.16794624071744985No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT24280.1667785163443633No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23560.16183286017599668No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23480.16128334282395596No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA23120.15881051473977262No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGTGAGCTACCATACTG21860.15015561644513103No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21720.14919396107905974No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC21620.14850706438900885No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC21620.14850706438900885No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA21280.1461716156428357No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20730.14239368384755563No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20180.13861575205227558No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA19890.13662375165112792No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC19450.13360140621490388No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGC18700.12844968103952198No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT18420.1265263703073794No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18100.12432830089921647No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT17960.12336664553314516No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17840.12254236950508407No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17830.12247367983607897No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG17760.12199285215304333No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17270.11862705837179383No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17100.11745933399870725No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCTCTGAACCTGTCTGGGATGCCA17030.11697850631567162No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17030.11697850631567162No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15870.10901050471108095No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15800.10852967702804531No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15580.10701850430993329No Hit
GGTTCAGAGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAACAGA15310.10516388324679579No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15190.10433960721873468No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15160.10413353821171942No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT15030.10324057251465321No Hit
TCTATGGTGCATCCGGCAGGGCCACTGGCATCCCAGACAGGTTCAGAGGCAGTGGGTCTGGGACAGACTTCACT14620.10042429608544444No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC14570.10008084774041899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)