FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871296
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26840.3080468635228441No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23840.27361539591596884No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20390.2340192081680623No Hit
CACCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGCCAGAGTCTCTGG20270.2326419494637873No Hit
TTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGA19200.22036139268400176No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19140.21967276333186425No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16900.1939639341853974No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGACTTCACACTGAA16180.1857003819597473No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15730.18053566181871603No Hit
CAGTAATAAAGTCCAACATCGTCAGCCTCAACCCTGCTAATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCA15620.17927317467313061No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15570.17869931687968268No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14580.16733693256941384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14520.16664830321727633No Hit
GTGCAGCCACAGTTCGTGTCAAATGCACCCTGGTCCCTCCGCCGAAAGTG13820.15861429410900543No Hit
GTCTGGGACTCCAGAGGCCCGAAAGGAAACCTTCTTGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA13790.15826997943293666No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13340.15310525929190538No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGATGAGATTGTGATGA13280.1524166299397679No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13120.15058028500073453No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTCAAATGCACCCTGGTCCCTCCGCCGAAAGTGAGGGGAAACT12750.14633373732921992No Hit
ATGTTGGACTTTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGACCAGGGTGCATTTGA12510.1435792199206699No Hit
CATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCAAGAAGGTTTCCTTTCGGGCCT12450.1428905905685324No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC12400.14231673277508447No Hit
GTAATAAAGTCCAACATCGTCAGCCTCAACCCTGCTAATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCA12130.1392179006904657No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12100.13887358601439695No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12080.1386440428970178No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11980.13749632731012193No Hit
GGTCTAGCCAGAGTCTCTGGGATGGTGATGAAGCAGAGACCCATTTGGACTGGTACCTGCAGAAGCCAGGGCAGT11800.13543043925370943No Hit
GCTAATGCTCTGGGTCCCTGGATCCAGTGATGAGATTGTGATGACGCAGACGCCACTCTCCCTGCCCGTCACAC11760.13497135301895108No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCAAGAAGGTTTCCTTTCGGGCC11700.13428272366681357No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11600.13313500807991774No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11520.13221683561040104No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11130.12774074482150727No Hit
GGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG10720.12303511091523432No Hit
CCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10690.12269079623916557No Hit
GGGTTGAGGCTGACGATGTTGGACTTTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGA10350.11878856324371971No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10300.11821470545027178No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10270.11787039077420303No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10020.11500110180696342No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGATGAGATTGTGATGACGCAGACGCCACTCTCC9700.11132841192889671No Hit
CTTTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGACCAGGGTGCATTTGACACGAA9670.11098409725282797No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9660.1108693256941384No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9560.10972161010724255No Hit
GCCACAGTTCGTGTCAAATGCACCCTGGTCCCTCCGCCGAAAGTGAGGGG9540.10949206698986338No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9510.10914775231379462No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9390.10777049360951962No Hit
CTCCCTGCCCGTCACACCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTA9180.10536029087703834No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT9130.10478643308359041No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9110.10455688996621126No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9090.10432734684883208No Hit
CCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9020.10352394593800499No Hit
GTCCAAATGGGTCTCTGCTTCATCACCATCCCAGAGACTCTGGCTAGACC8830.10134328632290289No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGATGAGATTGTGATGACGCAGACGCCACT8770.1006546569707654No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8760.10053988541207581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT2000.052.09292644
CGTATGC2100.049.38008541
TCGTATG2100.049.27341540
ACGAGAC2550.045.22802722
GGTATCA10650.044.267421
ATCTCGT2050.043.5064137
CCTAACG405.5336703E-542.391542
CTAACGC405.5336703E-542.391543
ATCTCCG2750.041.91705710
CCGTTAA751.031367E-940.69120816
CCCACGA2850.040.46253619
CGAGACC2800.039.98293723
CACGAGA2900.039.76490821
CATCTCG2250.039.58171536
GCCGTCT2600.038.8820546
GCCGTTA801.9426807E-938.14581715
ACCTCTC2950.037.9935427
TATACAC3600.037.681373
GTATGCC2800.037.08062442
TCTACAT3000.036.38927532
Produced by FastQC (version 0.11.5)