FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871296
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26890.30862072131629203No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24520.2814198619068606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20970.2406759585720582No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19590.22483748347289556No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18570.21313078448655798No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18510.21244215513442047No Hit
TTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGA18480.21209784045835167No Hit
CACCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTAGCCAGAGTCTCTGG16740.19212758924636403No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16020.18386403702071397No Hit
CAGTAATAAAGTCCAACATCGTCAGCCTCAACCCTGCTAATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCA15560.1785845453209931No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGCACTGACTTCACACTGAA14810.16997667841927427No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14700.16871419127368886No Hit
GTCTGGGACTCCAGAGGCCCGAAAGGAAACCTTCTTGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA13880.15930292346114294No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13780.1581552078742471No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC13090.1502359703246658No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTCAAATGCACCCTGGTCCCTCCGCCGAAAGTGAGGGGAAACT13060.14989165564859702No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12860.14759622447480536No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGATGAGATTGTGATGA12850.14748145291611578No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12770.14656328044659908No Hit
GTGCAGCCACAGTTCGTGTCAAATGCACCCTGGTCCCTCCGCCGAAAGTG12680.14553033641839283No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12580.144382620831497No Hit
GTAATAAAGTCCAACATCGTCAGCCTCAACCCTGCTAATTTTCAGTGTGAAGTCAGTGCCTGACCCACTGCCA12330.14151333186425738No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12090.13875881445570737No Hit
GGTCTAGCCAGAGTCTCTGGGATGGTGATGAAGCAGAGACCCATTTGGACTGGTACCTGCAGAAGCCAGGGCAGT12080.1386440428970178No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11850.13600429704715733No Hit
ATGTTGGACTTTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGACCAGGGTGCATTTGA11600.13313500807991774No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11480.13175774937564272No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11470.13164297781695314No Hit
GGGTTGAGGCTGACGATGTTGGACTTTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGA11140.12785551638019685No Hit
CATTTGGACTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCAAGAAGGTTTCCTTTCGGGCCT10980.1260191714411635No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10900.12510099897164684No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10860.12464191273688849No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10830.12429759806081975No Hit
GGACTCCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGG10750.12337942559130306No Hit
GCTAATGCTCTGGGTCCCTGGATCCAGTGATGAGATTGTGATGACGCAGACGCCACTCTCCCTGCCCGTCACAC10730.1231498824739239No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10690.12269079623916557No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCAAGAAGGTTTCCTTTCGGGCC10640.12211693844571764No Hit
CTTTATTACTGCATGCAATGGAGAGAGTTTCCCCTCACTTTCGGCGGAGGGACCAGGGTGCATTTGACACGAA9900.1136238431026884No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9630.11052501101806962No Hit
CCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9470.10868866607903628No Hit
CTCCCTGCCCGTCACACCTGGAGAGTCGGCCTCCATCTCCTGCAGGTCTA9460.10857389452034671No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCTGGATCCAGTGATGAGATTGTGATGACGCAGACGCCACTCTCC9270.1063932349052446No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9220.10581937711179668No Hit
CCTCAGTTCACCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG9140.10490120464228No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8890.10203191567504039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)