FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1465243
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAATCCGGGGTCCCT92590.6319088369642442No Hit
GTTTAGGAGGCTGTCCTGATTTCTGCTGATACCAAGCTAAGTAGTTTAAG72570.49527621015763257No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68990.47084340276664005No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA60510.41296904336004336No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53190.3630114595326509No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53170.36287496340197495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC51400.35079505583715465No Hit
GGCCAAAAGTGTGCGGAGGACTACCATAAAATTGCTGACAGAAATAAACTGCCACATCCTCGGCCTGCAGGCTG48840.3333235511106349No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42380.2892353009023077No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37410.25531601242933766No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37090.2531320743385227No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTGCGGAGGACTACCATAAAATTG34230.23361312765186387No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34220.2335448795865259No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34120.23286239893314625No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34100.23272590280247032No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32340.22071424330298797No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCGCTCTCAC32230.2199635145842703No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31890.2176430803627794No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30140.20569966892863503No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29790.2033109866418062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29300.19996683144024577No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28800.19655442817334734No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28790.1964861801080094No Hit
ATTTTATACAATTCCAACAACTTAAACTACTTAGCTTGGTATCAGCAGAA28340.19341501716780082No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27070.18474751286987892No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26240.17908292344682758No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25590.17464679919985968No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC25570.17451030306918375No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25290.17259935723972067No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC25260.17239461304370673No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT24840.16952819429951207No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24660.16829972912342867No Hit
CACCATCAGCAGCCTGCAGGCCGAGGATGTGGCAGTTTATTTCTGTCAGCAATTTTATGGTAGTCCTCCGCACA24610.16795848879673886No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24380.1663887832939656No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23820.16256689163503937No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAATATTTTATACAATTCCAA23550.16072419387091424No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCGCTCTCACCATCAGCAGCC22880.15615157349327038No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22100.1508282243969089No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGGAAATGAGCAGTTTAGGAGGCTGTCCTGATTTCTGCTGATA21860.14919027082879768No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21690.14803005371805222No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC21040.14359392947108432No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20900.14263845655635277No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20830.142160720098987No Hit
AATCAGGACAGCCTCCTAAACTGCTCATTTCCTGGGCATCTACCCGGGAA20810.14202422396831105No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20630.14079575879222764No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20510.13997678200817204No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19990.1364278826105977No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18860.12871585122740734No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18560.1266684092672683No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18100.12352899826172177No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCGGAATATTTTATACAATTCCAACAACTTAAACTA18060.1232560060003699No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18010.12291476567368007No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17860.12189104469361055No Hit
GATTTCGCTCTCACCATCAGCAGCCTGCAGGCCGAGGATGTGGCAGTTTATTTCTGTCAGCAATTTTATGGTAGT17810.12154980436692071No Hit
GTTTATTTCTGTCAGCAATTTTATGGTAGTCCTCCGCACACTTTTGGCCA17720.12093557177887901No Hit
GTCTGGGACAGATTTCGCTCTCACCATCAGCAGCCTGCAGGCCGAGGATG17610.12018484306016136No Hit
GGTCTGGGACAGATTTCGCTCTCACCATCAGCAGCCTGCAGGCCGAGGAT17480.11929761821076777No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17000.11602171107454533No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16850.1149979900944758No Hit
GGCCGAGGATGTGGCAGTTTATTTCTGTCAGCAATTTTATGGTAGTCCTC16740.11424726137575815No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16690.11390602104906832No Hit
GAAATAAACTGCCACATCCTCGGCCTGCAGGCTGCTGATGGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCA16600.11329178846102661No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16470.11240456361163301No Hit
GTCCTGATTTCTGCTGATACCAAGCTAAGTAGTTTAAGTTGTTGGAATTG16410.11199507521960522No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16390.11185857908892927No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16220.11069836197818382No Hit
TCCTCGGCCTGCAGGCTGCTGATGGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC16100.10987938519412821No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15870.10830967969135494No Hit
TCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG15750.10749070290729933No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15720.10728595871128542No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15390.10503377255513249No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15190.10366881124837313No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15180.10360056318303518No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15080.10291808252965548No Hit
GCTGATGGTGAGAGCGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT15020.10250859413762768No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14990.10230384994161378No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14890.1016213692882341No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14880.10155312122289613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)