FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154153
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA27970.2423422197923499No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27840.24121585266424816No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26080.22596657462225544No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA21210.18377112913105975No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGAGCTGGATCCG19880.17224752697432663No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG18480.16011741944092334No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT18470.16003077581568476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17960.1556119509285164No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17390.1506732642899165No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTT17180.148853748159906No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16300.14122910913890965No Hit
GTTTATTACTGTGCGAGAGGAGGCGTTCTGGGGGGGCAGCTGGTAGCGTT15680.13585720437411677No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15540.13464419362077645No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14770.12797263447740465No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14460.1252866820950082No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCGGTCACCTTCGGCCAAGGGA14220.12320723508928193No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT14120.12234079883689597No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14020.12147436258451003No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13980.12112778808355563No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13880.12026135183116969No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13260.11488944706637681No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13090.11341650543732071No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13060.11315657456160491No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12880.11159698930731021No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12870.11151034568207162No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA12820.11107712755587865No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA12790.11081719668016286No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12590.10908432417539096No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12420.10761138254633483No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT12410.10752473892109626No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGA12360.10709152079490328No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG12270.10631172816775593No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12240.10605179729204013No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11590.10041996165153146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAT11000.044.5743670
GGTATCA13300.044.016661
ACCGTAC2750.038.19589227
TCGTATG2950.037.3333540
GACCGTA2850.036.84129726
CCTAACG853.592504E-935.8237342
CTAACGC853.594323E-935.822183
CGTATGC3100.035.61482241
GTATCAA30450.034.117221
ACGAGAC3300.033.8320622
CACGAGA3400.033.8291321
GCCGTCT3100.032.57535646
CGTACTA3350.032.41129729
AGACCGT3500.031.91401325
ATGCCGT3300.031.52973744
GTACTAG3450.031.49373430
ATCTCGT2950.031.31366537
GAGACCG3650.030.59319324
CCCACGA3800.030.26817119
ATCTCCG3800.030.26292610
Produced by FastQC (version 0.11.5)