FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154153
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32670.2830647236544895No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28210.24442166679807617No Hit
GTAATAAACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA27220.2358439478994553No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22180.19217556077920345No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA20280.17571327198387043No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGAGCTGGATCCG19290.16713555308524952No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT17130.14842053003371303No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16930.14668765752894114No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16710.14478149777369204No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16680.14452156689797627No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16610.1439150615213061No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG16460.1426154071427272No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15940.13810993863032026No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTGGCCCTGTT15650.13559727349840098No Hit
GTTTATTACTGTGCGAGAGGAGGCGTTCTGGGGGGGCAGCTGGTAGCGTT14530.12589318747167835No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14500.12563325659596258No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT14050.12173429346022582No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13700.11870176657687499No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13570.11757539944877325No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13410.11618910144495574No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13290.1151493779420926No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA13030.11289664368588914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12830.11116377118111723No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG12710.11012404767825411No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA12580.10899768055015237No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCGGTCACCTTCGGCCAAGGGA12500.10830453154824361No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12190.10561857916584717No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12000.10397235028631385No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGTTACTACTGGA11920.1032792012844051No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11780.10206619053106478No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11770.10197954690582618No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11600.10050660527677005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG752.1827873E-1145.0931322
CTAACGC752.1827873E-1145.0735863
TACTGAT11750.042.061170
GGTATCA12100.039.7031821
GTATCAA31900.038.922231
TATCAAC37450.032.781322
ATCAACG37550.032.769883
TCAACGC37900.032.1926884
AACGCAG38450.031.9093786
CAACGCA38500.031.6923585
CGCAGAG40700.029.8962128
ACGCAGA41500.029.4827867
AGAGTAC41950.028.92481211
TTCGATC1353.237801E-1027.98282239
CCGTGTC10200.027.83232912
GCAGAGT43700.027.7664979
CTAAAGT757.089704E-527.0382924
TGCGGCG10650.026.36068219
CGGCGGT11000.026.13918521
TAAACGG11000.026.117665
Produced by FastQC (version 0.11.5)