FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678724
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT160250.9545940845546975No Hit
CTATTATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCAT134870.8034078264205432No Hit
GTTTTATACAGCCCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACT92000.5480352934728996No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61120.3640860558376481No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58460.3482406875698447No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45820.2729454037709594No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA45420.27056264162542504No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35740.21289979770349382No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34490.205453665998699No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34470.2053345278914223No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGGCA34460.20527495883778396No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33840.20158167751220568No Hit
GTCAAAGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA33230.1979479652402658No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32280.19228890514462174No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32040.19085924785730116No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30980.18454492817163512No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29560.1760861225549882No Hit
ATTCAGTGCCAGCGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGCC29150.17364379135581548No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGCGACAGATTTCACTCTCAC28990.17269068649760175No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28930.1723332721757716No Hit
GTTTATTACTGTCAGCACTATTATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAA28860.1719162888003031No Hit
AACCAGGACAGCCTCCTAAGCTACTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG28350.1688782670647468No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28300.168580421796555No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27730.16518498573916857No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA27730.16518498573916857No Hit
GTGCAGCCACAGTTCGTTTGATCTGCAGCTTGGTCCCCTGGCCAAAACTG27190.1619682568426972No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA27010.16089601387720673No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC26450.15756014687345865No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26330.15684531822979833No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACAGCCCCAA25580.15237763920692143No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGC25510.15196065583145293No Hit
CTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG25490.15184151772417623No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25270.15053099854413232No Hit
GGCCAAAACTGAACGGAGCACTATAATAGTGCTGACAGTAATAAACTGCC25160.14987573895411038No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24940.14856521977406648No Hit
GTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCA24780.14761211491585274No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23750.1414765023911018No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22850.13611528756364955No Hit
CTCCTAAGCTACTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGTCTG22650.13492390649088237No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22550.1343282159544988No Hit
GACCGATTCAGTGCCAGCGGGTCTGCGACAGATTTCACTCTCACCATCAG22340.13307726582809323No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22210.1323028681307946No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21970.13087321084347397No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21920.13057536557528218No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT21860.13021795125345204No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21450.1277756200542793No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGGGCTGTATAAAACACT21400.12747777478608754No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCAGCTTGGTCC21250.12658423898151214No Hit
GAGCACTATAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT20840.12414190778233944No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGGCACTGAATCGGTCAGGGACC20690.12324837197776406No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTCAAAGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA20620.12283138860229555No Hit
GTGCCAGCGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTA20470.12193785279772018No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20460.12187828374408181No Hit
ATATATTACTGTGCGAAAAGTTGTAGTGGAGTAAATGATGGTTTTGATAT20440.12175914563680511No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGCTTTTT20160.12009121213493106No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19850.11824457147214193No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19700.11735103566756655No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19700.11735103566756655No Hit
ATTATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAA19510.11621922364843774No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACAGCCCCAACAATAAGAACTA18820.11210895894739101No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18820.11210895894739101No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGGGCTG18790.11193025178647593No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18580.1106793016600704No Hit
ATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGA18450.10990490396277172No Hit
AAACTGAACGGAGCACTATAATAGTGCTGACAGTAATAAACTGCCACATC18430.109785765855495No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18200.10841567762181276No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18030.10740300370996067No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCTTTGACTGGGCATCTACCCGGGAATC18030.10740300370996067No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACTCTTT18000.10722429654904557No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTAC17990.10716472749540723No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17880.10650946790538528No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17640.10507981061806466No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC17590.10478196534987289No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGGCA17520.10436498197440437No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17190.10239920320433854No Hit
AAGCTACTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCG16820.10019514821971925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)