FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004692314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678724
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT146830.8746524145720201No Hit
CTATTATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCAT130420.7768995975514736No Hit
GTTTTATACAGCCCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACT91340.544103735932768No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61880.36861330391416336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51030.3039808807165442No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46510.2770556684720061No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40850.24333958411269513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39000.23231930918959875No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA36060.21480600741992134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35640.21230410716711026No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35030.20867039489517036No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34910.20795556625151007No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGGCA33930.20211779899495094No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33040.196816153221137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31210.18591501640531738No Hit
GTTTATTACTGTCAGCACTATTATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAA29350.17483517242858265No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC29010.17280982460487845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28720.17108232204936608No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATA28430.16935481949385367No Hit
AACCAGGACAGCCTCCTAAGCTACTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTG28010.16685291924104262No Hit
GTCAAAGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA27840.1658402453291905No Hit
GTCCCTGACCGATTCAGTGCCAGCGGGTCTGCGACAGATTTCACTCTCAC27840.1658402453291905No Hit
GTGCAGCCACAGTTCGTTTGATCTGCAGCTTGGTCCCCTGGCCAAAACTG27410.1632787760227411No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA26990.16077687576993002No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26710.15910894226805597No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA26070.155296522835201No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25960.15464126324517907No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25960.15464126324517907No Hit
GTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGAACTGTGGCTGCACCA25920.15440298703062566No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACAGCCCCAA25470.1517223796168995No Hit
ATTCAGTGCCAGCGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGCC25410.15136496529506935No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24440.14558676709214857No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24300.14475280034121155No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24100.14356141926844437No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24030.14314443589297585No Hit
CTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG23740.14141693333746347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23600.14058296658652644No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGC23420.13951072362103598No Hit
GGCCAAAACTGAACGGAGCACTATAATAGTGCTGACAGTAATAAACTGCC23210.13825977349463045No Hit
GACCGATTCAGTGCCAGCGGGTCTGCGACAGATTTCACTCTCACCATCAG22820.13593658040273446No Hit
CTCCTAAGCTACTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGCCAGCGGGTCTG22340.13307726582809323No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22180.1321241609698795No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21660.12902657018068486No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21180.12616725560604364No Hit
CTATAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT21150.12598854844512855No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20900.1244993221041696No Hit
GAGCACTATAATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT20810.12396320062142435No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGGCACTGAATCGGTCAGGGACC20590.12265268144138047No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20290.12086560983222971No Hit
GTGCCAGCGGGTCTGCGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTA20100.11973379781310091No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGGGCTGTATAAAACACT19880.11842327863305702No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT19770.11776801904303508No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19690.1172914666139282No Hit
ATATATTACTGTGCGAAAAGTTGTAGTGGAGTAAATGATGGTTTTGATAT19600.11675534513118296No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTCAAAGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA19330.11514698068294728No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19280.1148491354147555No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19090.11371732339562667No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18930.11276421853741293No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18710.11145369935736905No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18550.11050059449915532No Hit
AAACTGAACGGAGCACTATAATAGTGCTGACAGTAATAAACTGCCACATC18300.10901136815819634No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA18210.10847524667545111No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18110.10787955613906752No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGGGCTG17960.10698602033449216No Hit
ATTATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAA17780.10591377736900169No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17730.10561593210080991No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGTTGGCTTTTT17530.10442455102804274No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17470.10406713670621257No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAGGTCCAGCCAGAGTGTTTTATACAGCCCCAACAATAAGAACTA17250.10275661752616869No Hit
AAGCTACTCTTTGACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCG17050.10156523645340151No Hit
ATAGTGCTCCGTTCAGTTTTGGCCAGGGGACCAAGCTGCAGATCAAACGA17030.1014460983461248No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17020.10138652929248644No Hit
GAGTGAAATCTGTCGCAGACCCGCTGGCACTGAATCGGTCAGGGACCCCG16990.10120782213157137No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCGCAGACCCGCTGGCA16960.10102911497065628No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16950.10096954591701793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)