FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253801
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGCTCCTTCAGTGACTACTCCTGGACCTGGATCCGCCAGCC37650.3002868876320884No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34050.27157419718121134No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAG32000.2552239151189064No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC30700.24485544356720085No Hit
GTACAGGGGCCTGCCCTGGCTTCTGCTGGTACCACGTTGCATAATAGGTG28770.22946225118659183No Hit
CCCCTGTACTTGTCAGCTTTGCTAGAGACACCCGGGCCTCAGGGATCCCA28680.2287444339253199No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25890.20649209882589023No Hit
CCAATAGACACCGGTGTCCGCGGCGGTCACAGAGCTCAGGTCCAGGGAGAACTGCTTGGTGGACGTGTCTACTGA24080.1920559961269771No Hit
CTCTTTACATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTACT23420.18679200287764966No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAGATGGTAACCA23060.18392073383256194No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22890.18256485678349277No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20210.16118985389228435No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19910.15879712968804457No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19720.15728173769202608No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19530.15576634569600759No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT19430.154968770961261No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18080.14420151204218212No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17390.13869824637243072No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17240.13750188427031085No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17080.1362257646947163No Hit
ACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGCACCTATTATG16860.13447110027827383No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16460.1312808013392875No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16280.12984516681674366No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16260.12968565186979433No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16250.12960589439631967No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15690.12513947588173882No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15530.12386335630614428No Hit
CTCTGAGAGTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC15520.12378359883266962No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15270.12178966199580316No Hit
GTCTATTATTGTAATTCTCGGGACAGCAGTGGTTACCATCTGGTCTTCGG15230.12147063210190452No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15210.12131111715495521No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14990.11955645273851274No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14920.11899815042419011No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14890.11875887800376615No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14720.11740300095469695No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14490.11556857906477981No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC14280.11389367212181198No Hit
GTCTATTGGTGTGCGAGAGGTTTCAAGTCTTCCTGGGTGGGAGCACACAG14130.11269731001969212No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14000.11166046286452157No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13940.11118191802367361No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTATGCGGCCAGCAGCTAC13770.10982604097460441No Hit
GGACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCC13640.10878919381943387No Hit
GCATAATAGGTGCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTG13540.10799161908468728No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13490.10759283171731399No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13400.10687501445604207No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13280.10591792477434618No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13270.1058381673008715No Hit
ACCATAGACAGCGCACGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTCCTGCGCCCCGCTGCTGTAGCTGCAC13220.10543937993349821No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12930.10312641320273314No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC12930.10312641320273314No Hit
GTTATACCTCCTGTGTGCTCCCACCCAGGAAGACTTGAAACCTCTCGCAC12900.10288714078230915No Hit
ATAATAGACAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG12680.10113247636586667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12640.10081344647196805No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12590.10041465910459474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA4200.049.0322538
CGCTAAT5200.048.4659532
TCGTATG4700.048.3714940
AATCTCG4300.047.7473636
CGTATGC4650.047.5101741
TCGCTAA5300.047.50391431
GCTAATC4600.047.45041333
ATCTCGT4350.047.2632337
ACTCGCT5450.046.75337629
CACTCGC5450.046.73282628
ATGCCGT4400.046.55165544
GGTATCA12750.045.740341
CTCGCTA5600.045.53563330
ACACTCG5700.044.6510327
TGGATAG14050.043.83921470
GCCGTCT4300.043.76368346
TATGCCG4900.043.11634443
GTATGCC5250.042.80051442
CGAGACA5950.042.7391123
ATCTCCG6250.041.76097510
Produced by FastQC (version 0.11.5)