FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253801
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50210.40046227431625914No Hit
GTCTATGGTGGCTCCTTCAGTGACTACTCCTGGACCTGGATCCGCCAGCC38110.3039557314119226No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC32640.26032839342128455No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31030.24748744019186456No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAG28610.2281861316109973No Hit
GTACAGGGGCCTGCCCTGGCTTCTGCTGGTACCACGTTGCATAATAGGTG27500.21933305205531023No Hit
CCCCTGTACTTGTCAGCTTTGCTAGAGACACCCGGGCCTCAGGGATCCCA27330.21797717500624103No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24830.19803780663757647No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24280.19365114559647026No Hit
CTCTTTACATAGGTTCTGTGGTTTCTTCTGAACTGACTCAGGACCCTACT22830.18208631194264482No Hit
CCAATAGACACCGGTGTCCGCGGCGGTCACAGAGCTCAGGTCCAGGGAGAACTGCTTGGTGGACGTGTCTACTGA21900.1746688669095016No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21640.1725951725991605No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19460.15520804338168498No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAGATGGTAACCA19300.15393192380609044No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18700.14914647539761094No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18120.14452054193608074No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18110.1444407844626061No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16830.13423182785784984No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16600.1323974059679327No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16310.13008443923716762No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16240.129526136922845No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16230.12944637944937035No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16180.12904759208199706No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16140.12872856218809842No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15650.12482044598784017No Hit
CTCTGAGAGTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC15230.12147063210190452No Hit
ACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCCAAGGAGACAGCCTCAGCACCTATTATG15220.12139087462842987No Hit
GTCTATTATTGTAATTCTCGGGACAGCAGTGGTTACCATCTGGTCTTCGG14950.11923742284461411No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14820.11820057568944355No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14810.11812081821596888No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14360.11453173190960925No Hit
GTCTATTGGTGTGCGAGAGGTTTCAAGTCTTCCTGGGTGGGAGCACACAG14350.11445197443613461No Hit
GGACCCTACTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCACATGCC14060.11213900770536951No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14040.11197949275842019No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13720.10942725360723114No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13670.10902846623985785No Hit
GCATAATAGGTGCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTG13510.10775234666426331No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13410.10695477192951672No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13410.10695477192951672No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG13290.10599768224782082No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13220.10543937993349821No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13120.10464180519875164No Hit
ACCATAGACAGCGCACGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTCCTGCGCCCCGCTGCTGTAGCTGCAC12960.10336568562315711No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)