FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504543
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58650.38981936707691306No Hit
GTGATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATATAGTGTGTTCTTGGAATTGTCTCTGGA50890.338242243658041No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG45500.3024174117987987No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA35170.23375868951568685No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG31280.2079036624410203No Hit
GTATTACTGTCAGCAGTATGGTAGCTCGGTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG30370.20185531420504432No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT27240.18105165488789618No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTTAGCAGCTATGGCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG25010.16622987844149353No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC24590.16343833310181233No Hit
GTATATCACTGTGCGAAAGTGGTGGGCAGTAACTGGTACTTTCCCTTTGA24130.16038092630120906No Hit
GTGTGAGGTGCAGCTGTTGCAGTCTGGGGGAGGCTTGGTACAGCCTGGGG23850.15851989607475495No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23400.15552895463938218No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22670.15067698297755533No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21910.14562561522003692No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19460.12934160073856313No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC19120.12708177832072598No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18970.1260847978422684No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC18300.12163161837182453No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT17950.11930533058875685No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC17900.11897300376260433No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG17870.1187736076669128No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT17450.11598206232723159No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC17080.1135228438137029No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16880.11219353650909279No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC16590.11026604091740815No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16340.10860440678664551No Hit
GTACCGGCCTGGGGCCTGGGGCTCAGGCATTGGGGCGCTGGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCT15670.10415122731620167No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT15650.10401829658574066No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT15570.1034865736638966No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15460.10275545464636104No Hit
CACTATATCTGCAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTATAT15420.10248959318543904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.044.8395651
CTAACGC705.0749804E-1043.7514723
GTATCAA39800.039.524561
CCTAACG801.877197E-938.283812
TATCAAC44100.035.4190372
ATCAACG45050.034.5199053
TCAACGC45050.034.5199054
TCGATCC807.1193426E-834.48750340
CAACGCA46150.033.7708475
TACGTAT1052.2216095E-433.47453370
AACGCAG47500.032.9554186
TTCGATC958.976713E-932.61742439
CGCAGAG49450.031.6569168
AGAGTAC50150.031.07932511
CGGACGA450.004947920330.24390814
GCAGAGT51900.030.1625149
AGATATA21300.029.7836344
CAGAGTA52700.029.70463810
CGGCTCT19750.029.48261621
CGGAAAT1052.5705958E-829.27664830
Produced by FastQC (version 0.11.5)