FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412975
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT15540.3762939645256977No Hit
GTCAATAACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGATATAACTATGTCTCCTGGTACCAACAGCA15470.37459894666747384No Hit
CTGTAGCTGCCTGCATATGCGCAGCAGTAATAAGCAGCCTCATCTTCAGC12170.29469096192263455No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11120.2692656940492766No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCCCCACCAAATAGCACACTGTAGCTGCCTGCATATGCGCAG10580.25618984200012107No Hit
GCTTATTACTGCTGCGCATATGCAGGCAGCTACAGTGTGCTATTTGGTGGGGGGACGAAGCTGACCGTCCTAGGT9710.23512319147648164No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9470.22931170167685694No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9420.22810097463526846No Hit
GTGTCCGGGTCTCCTGGACAGTCAATAACCATCTCCTGCACTGGAACCAG9130.2210787577940553No Hit
GACCTAGGACGGTCAGCTTCGTCCCCCCACCAAATAGCACACTGTAGCTGCCTGCATATGCGCAGCAGTAATAAG8480.20533930625340518No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCCC7950.19250559961256736No Hit
GTAATATACGGCCGTGTCGTCGACTCTCAGGTTGTTCATTTGCAGAAACA7420.17967189297172953No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT7330.1774925842968703No Hit
GTATATTACTGTGCGAGAGATTTAAGCTCTCTGGGTGGTGGTAGCTGTTA7270.1760397118469641No Hit
CAGCTACAGTGTGCTATTTGGTGGGGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA7270.1760397118469641No Hit
GTAATAAGCAGCCTCATCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG7190.17410254858042254No Hit
TCTCTGGGCTCCAGGCTGAAGATGAGGCTGCTTATTACTGCTGCGCATAT6550.15860524244809007No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6500.1573945154065016No Hit
CTCCTGGACAGTCAATAACCATCTCCTGCACTGGAACCAGCAGTGATGTT6360.15400447969005387No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6300.1525516072401477No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6280.15206731642351232No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAATAACCATCTCCTG6150.14891942611538228No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5990.14504509958229916No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5930.143592227132393No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG5840.14141291845753376No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG5790.14020219141594528No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG5690.13778073733276833No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG5670.13729644651613293No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT5610.13584357406622677No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAATAAC5600.13560142865790908No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5550.1343907016163206No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5290.12809492100006054No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC5230.12664204855015435No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5190.12567346691688358No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA5080.12300986742538894No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC4960.1201041225255766No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA4880.11816695925903505No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4730.11453477813426964No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC4730.11453477813426964No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC4530.10969186996791574No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT4440.10751256129305647No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG4430.10727041588473878No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC4380.10605968884315031No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4220.10218536231006721No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC4190.1014589260851141No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4150.10049034445184332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)