FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616891
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACAC63730.3941514919682279No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54200.3352112170826605No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACACATAAGTACCA52140.3224707169499985No Hit
ATAATATACAGCCGCGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAAA49950.30892620467304227No Hit
ATATAAAGACAATGCGAGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCT46580.2880837360094156No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41530.2568509565579869No Hit
GTCTTTATATATCACCATCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC41170.25462446138917216No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40230.24881083511504487No Hit
GTATATTATTGTGCGAGACATTTAAGTCCCGGGGGAGGGACGATGGGAGC39280.2429353617528949No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC39220.24256427922475912No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38890.24052332532001228No Hit
GTCTCTGGTGGCTCCATGAGCAGTAGTAGTTACTACTGGGGCTGGATCCG38880.24046147823198966No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAC37700.23316352184531922No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32480.20087934189750578No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG32250.19945685887298525No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32180.19902392925682683No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31440.19444724474315214No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG31280.19345769133479004No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC30690.1898087131414548No Hit
GACTATTACTGTCAATCAGCAAACAGCCGTGGTACTTATGTGTTTTTCGG30160.18653081747625536No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28880.17861439020935857No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26750.16544096046053816No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT26650.16482248958031184No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACACATAAGTACCAC26460.16364739490788185No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26310.16271968858754238No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25900.1601839579786145No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25850.15987472253850135No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGAGA24290.1502265768069709No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24180.14954625883872197No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC24030.1486185525183825No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23900.14781454037408828No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23670.14639205734956778No Hit
GGCCAGGCCCCTGTGATGGTGATATATAAAGACAATGCGAGGCCCTCAGG23620.14608282190945462No Hit
CACCTGCACTGTCTCTGGTGGCTCCATGAGCAGTAGTAGTTACTACTGGG23490.14527880976516042No Hit
CTATTACTGTCAATCAGCAAACAGCCGTGGTACTTATGTGTTTTTCGGCG23400.14472218597295672No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23290.1440418680047078No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22830.14119690195566675No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22220.13742422958628628No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG22100.1366820645300147No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21920.13556881694560735No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC21870.13525958150549416No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCTCGTTGACCATCAGTGGAGTCCAGG21630.13377525139295104No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21530.13315678051272473No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21160.13086843825588737No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21150.13080659116786475No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20930.12944595523136687No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20740.12827086055893688No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20470.12660098918232582No Hit
CAATAATATACAGCCGCGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGA19960.12344678769317165No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA19920.12319939934108114No Hit
GTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTG19770.12227169302074165No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19410.12004519785192695No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19300.119364879883678No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18990.11744762015497644No Hit
CTATTATAGTGAGACTTCCTACTACAATTCGTCCCTCAAGAGTCGAGTCA18960.11726207889090855No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18780.1161488313065012No Hit
CCCCTGAGGGCCTCGCATTGTCTTTATATATCACCATCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAG18700.11565405460232012No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18550.11472634828198067No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18390.11373679487361858No Hit
GCTCTGGAGATGCATTGCCAAACCAATATGCTTATTGGTACCAGCAGAAG18050.1116339938808491No Hit
CCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGATGGTGATATATAAAGACAATGCGAGGCC17920.11082998173655491No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17820.1102115108563286No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17600.10885087491983071No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17290.10693361519112915No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACAGTGACCATTGTCCCTTGG17280.10687176810310652No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17180.10625329722288021No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17150.10606775595881231No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT17140.10600590887078969No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17110.10582036760672178No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17060.10551113216660864No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT16880.10439788458220128No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16770.10371756661395233No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16670.10309909573372601No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16400.10142922435711497No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16180.1000685884206171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT16250.055.70289670
CCGACGC200.006799867452.7955557
GGTATCA11950.046.654961
GTGGTAT3700.042.524011
TGGTATC3600.041.7985422
TACGTAT857.41617E-540.33743770
GTATCAA29900.037.4071541
AGTATCG400.00192179836.6822667
CGTATGC952.1827873E-1036.3324841
CTACGGA16750.036.0836669
AGTAATA24050.033.36015370
TCGTATG1056.4574124E-1032.84558540
TATCAAC35700.031.037572
ATCAACG35700.030.9417763
GCTGATT30400.030.67768570
CGCTAAT902.108045E-730.4762332
TCAACGC36300.030.4312864
CAACGCA37050.029.9075765
AATGGTA6200.029.86271570
CGTGTCT17250.029.82907964
Produced by FastQC (version 0.11.5)