FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1616891
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACAC60350.3732471762165786No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50980.3152964547393733No Hit
ATAATATACAGCCGCGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGAAA46810.28950621903393614No Hit
ATATAAAGACAATGCGAGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCT45580.2818990272071525No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45180.27942514368624727No Hit
GTCTTTATATATCACCATCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC43770.2707047042750563No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACACATAAGTACCA40900.25295459001256115No Hit
GTCTCTGGTGGCTCCATGAGCAGTAGTAGTTACTACTGGGGCTGGATCCG39450.24398676224927965No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38860.24033778405594441No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38830.24015224279187652No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC37260.2304422499723234No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAC36520.22586556545864875No Hit
GTATATTATTGTGCGAGACATTTAAGTCCCGGGGGAGGGACGATGGGAGC36480.22561817710655818No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33200.2053323322351352No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG32070.19834361128857786No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC31140.1925918321024732No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29170.18040795576201488No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG29140.180222414497947No Hit
GACTATTACTGTCAATCAGCAAACAGCCGTGGTACTTATGTGTTTTTCGG28550.17657343630461175No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27430.16964656244607706No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG27370.16927547991794129No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26550.16420401870008552No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26410.1633381594677687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26360.16302892402765554No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25640.15857593369002612No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24970.1544321787925098No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24820.15350447247217036No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC24640.152391224887763No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24390.15084504768719723No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23880.14769084619804304No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23550.14564989229329622No Hit
CTATTACTGTCAATCAGCAAACAGCCGTGGTACTTATGTGTTTTTCGGCG23050.14255753789216466No Hit
GGCCAGGCCCCTGTGATGGTGATATATAAAGACAATGCGAGGCCCTCAGG22790.14094951360357624No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22440.13878486552278416No Hit
CACCTGCACTGTCTCTGGTGGCTCCATGAGCAGTAGTAGTTACTACTGGG22350.1382282417305805No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22290.13785715920244468No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAAACACATAAGTACCAC22070.13649652326594683No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22060.13643467617792418No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22010.13612544073781102No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC21860.13519773441747154No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21600.13358971012888315No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20960.12963149649543476No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20950.12956964940741214No Hit
CCTCAGGGGTCCCTGAGCGATTCTCTGGCTCCAGCTCAGGGACAACAGTCTCGTTGACCATCAGTGGAGTCCAGG20800.12864194308707266No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA19770.12227169302074165No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT19580.12109659834831166No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC19450.12029258620401746No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19380.11985965658785905No Hit
CAATAATATACAGCCGCGTCTGCGGCGGTCACAGAGGTCAACTTCAGGGA18740.11590144295441066No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18700.11565405460232012No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGAGA18440.11404603031373171No Hit
CCCCTGAGGGCCTCGCATTGTCTTTATATATCACCATCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAATAAG18280.11305647690536963No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18270.11299462981734701No Hit
GCTCTGGAGATGCATTGCCAAACCAATATGCTTATTGGTACCAGCAGAAG18220.11268539437723384No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC17790.1100259695922607No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17780.10996412250423807No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17760.10984042832819282No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17370.10742839189531021No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17320.10711915645519705No Hit
CTATTATAGTGAGACTTCCTACTACAATTCGTCCCTCAAGAGTCGAGTCA17290.10693361519112915No Hit
GTAGTAGTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTG17260.10674807392706126No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACAGTGACCATTGTCCCTTGG17240.10662437975101598No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17160.10612960304683494No Hit
CCAATATGCTTATTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGATGGTGATATATAAAGACAATGCGAGGCC16910.10458342584626916No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT16900.10452157875824654No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAATAAGCATATTGGTTTGGCAATGCATCTCCAGAGCAGGTGATCCTGGC16650.10297540155768076No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG16520.10217138941338655No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16410.10149107144513761No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16330.10099629474095656No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16310.1008726005649113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)