FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250772
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47890.3828835311311734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40250.32180125554457567No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33550.26823433847255934No Hit
GTCCCAACCAGGACGCAGCATGGACACGAGGGTCCCTGCTCAGCTCCTGG31940.25536228825077634No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28160.22514095294745964No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC27460.2195444093727714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27110.21674613758542724No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25810.20635255666100616No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24340.19459981515416078No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24260.1939602101741964No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23200.18548544418966845No Hit
CTTGGGGACAGAGTCACCATCACTTGCCAGGCGAGTGAAGACGTTGGCGACTCTGTCAATTGGTATCAGCAACGG22960.18356662924977535No Hit
GACTTCAGCCTCACCATTGACGACGTGAGGTCTGAGGATGCTGCAACTTA21860.1747720607752652No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20950.16749655412817044No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20860.1667769985257105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20730.1657376404332684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20670.16525793669829514No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20240.16182005993098664No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19430.15534405950884733No Hit
GGCCAGGGGAGGTCCCGAGTCTCCTGGTCCACTCCTCTTCTCAGACCCAC19310.15438465203890078No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18060.14439082422695743No Hit
CTATTTGCAGTTCAGTTTTGGGCCCGGGACCAGAGTCGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCAT17740.14183240430709992No Hit
GTGCAGCCACAGTTCCTTTGATGTCGACTCTGGTCCCGGGCCCAAAACTGAACTGCAAATAGTGTTCCCACTG17310.13839452753979142No Hit
ACTTATTACTGTCAGCAGTGGGAACACTATTTGCAGTTCAGTTTTGGGCC17300.13831457691729587No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17140.13703536695736715No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATGTCGACTCTGGTCC16850.13471679890499627No Hit
ACATTGGGGTCTCTTCGAGATTCAGGGGTTCTGGATCCGGGACGGACTTCAGCCTCACCATTGACGACGTGAGGT16800.13431704579251855No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16770.1340771939250319No Hit
GCATGGACACGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15940.12744129225790152No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT15330.12256430428567316No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15220.12168484743822217No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15160.12120514370324888No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14880.11896652627337358No Hit
CCTCTTCTCAGACCCACATTGGGGTCTCTTCGAGATTCAGGGGTTCTGGA14740.11784721755843591No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14560.11640810635351606No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14360.11480909390360515No Hit
GTCTGAGGATGCTGCAACTTATTACTGTCAGCAGTGGGAACACTATTTGC14030.11217072336125208No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13770.11009200717636788No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13750.10993210593137678No Hit
CTCCTGGTCCACTCCTCTTCTCAGACCCACATTGGGGTCTCTTCGAGATTCAGGGGTTCTGGATCCGGGACGGA13590.10865289597144805No Hit
CCCCAATGTGGGTCTGAGAAGAGGAGTGGACCAGGAGACTCGGGACCTCCCCTGGCCGTTGCTGATACCAATTGA13530.10817319223647476No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13500.1079333403689881No Hit
TCCTCAGACCTCACGTCGTCAATGGTGAGGCTGAAGTCCGTCCCGGATCCAGAACCCCTGAATCTCGAAGAGACC13300.10633432791907718No Hit
GAGTCACCATCACTTGCCAGGCGAGTGAAGACGTTGGCGACTCTGTCAAT13200.10553482169412172No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12830.10257664866178647No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12590.10065783372189335No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12550.10033803123191117No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAA200.006674022453.0447757
CAACGGT400.00898713449.1958370
ATCGGGC350.001184901540.4754758
CGTATGC1200.040.31759641
TCGTATG1050.039.4500340
GTATCAA38100.037.6062621
TCTCCGA3050.033.53756311
CACGAGA2950.033.52821
TATCAAC43350.032.8865852
CCGCTAT1002.504594E-432.7972270
TATACTG959.844371E-932.309725
TCAACGC45950.031.4698454
ATCAACG45950.031.172963
AACGCAG46850.030.8653036
CAACGCA46950.030.799565
ATCGCGT450.00470531830.55598437
CTTATAC3700.030.4262261
TAACGGC1052.4356268E-829.43859936
ACGAGAC3250.029.38486322
CCCACGA3150.029.23143219
Produced by FastQC (version 0.11.5)