FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250772
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46530.372010246471779No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33130.2648764123277464No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32590.2605590787129869No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32400.2590400168855715No Hit
GTCCCAACCAGGACGCAGCATGGACACGAGGGTCCCTGCTCAGCTCCTGG30200.24145087993655118No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26670.21322831019562316No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24320.1944399139091697No Hit
CTTGGGGACAGAGTCACCATCACTTGCCAGGCGAGTGAAGACGTTGGCGACTCTGTCAATTGGTATCAGCAACGG23860.19076218527437455No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23710.18956292593694135No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23460.18756416037455267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22170.17725053007262714No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22020.17605127073519394No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21680.17333294957034537No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21470.17165398649793887No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20700.16549778856578176No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20260.1619799611759777No Hit
GACTTCAGCCTCACCATTGACGACGTGAGGTCTGAGGATGCTGCAACTTA20100.16070075121604896No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19520.15606361511130726No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18950.1515064296290611No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18630.14894800970920358No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18160.1451903304519129No Hit
CTATTTGCAGTTCAGTTTTGGGCCCGGGACCAGAGTCGACATCAAAGGAACTGTGGCTGCACCATCTGTCTTCAT17710.1415925524396133No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17540.140233391857189No Hit
GTGCAGCCACAGTTCCTTTGATGTCGACTCTGGTCCCGGGCCCAAAACTGAACTGCAAATAGTGTTCCCACTG17520.14007349061219793No Hit
GGCCAGGGGAGGTCCCGAGTCTCCTGGTCCACTCCTCTTCTCAGACCCAC17410.1391940337647469No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17020.13607595948742057No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16910.13519650263996955No Hit
ACATTGGGGTCTCTTCGAGATTCAGGGGTTCTGGATCCGGGACGGACTTCAGCCTCACCATTGACGACGTGAGGT16540.13223832960763432No Hit
ACTTATTACTGTCAGCAGTGGGAACACTATTTGCAGTTCAGTTTTGGGCC16350.13071926778021895No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15720.1256823785629995No Hit
CTCCTGGTCCACTCCTCTTCTCAGACCCACATTGGGGTCTCTTCGAGATTCAGGGGTTCTGGATCCGGGACGGA15500.12392346486809747No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14920.11928632876335575No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATGTCGACTCTGGTCC14720.11768731631344481No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14450.11552864950606506No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14060.11241057522873873No Hit
GTCTGAGGATGCTGCAACTTATTACTGTCAGCAGTGGGAACACTATTTGC14020.11209077273875655No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT14020.11209077273875655No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG13800.11033185904385451No Hit
CCTCTTCTCAGACCCACATTGGGGTCTCTTCGAGATTCAGGGGTTCTGGA13700.10953235281889905No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13630.10897269846143022No Hit
TCCTCAGACCTCACGTCGTCAATGGTGAGGCTGAAGTCCGTCCCGGATCCAGAACCCCTGAATCTCGAAGAGACC13580.1085729453489525No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13580.1085729453489525No Hit
GCATGGACACGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13340.10665413040905937No Hit
GTAATAAGTTGCAGCATCCTCAGACCTCACGTCGTCAATGGTGAGGCTGAAGTCCGTCCCGGATCCAGAACCC13260.10601452542909498No Hit
CCCCAATGTGGGTCTGAGAAGAGGAGTGGACCAGGAGACTCGGGACCTCCCCTGGCCGTTGCTGATACCAATTGA13230.10577467356160834No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13070.10449546360167959No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12910.10321625364175085No Hit
CTGCAAATAGTGTTCCCACTGCTGACAGTAATAAGTTGCAGCATCCTCAGACCTCACGTCGTCAATGGTGAGG12790.10225684617180429No Hit
GTGGGAACACTATTTGCAGTTCAGTTTTGGGCCCGGGACCAGAGTCGACATCAAAGGAACTGTGGCTGCACCAT12540.10025808060941561No Hit
CCCCTGGCCGTTGCTGATACCAATTGACAGAGTCGCCAACGTCTTCACTCGCCTGGCAAGTGATGGTGACT12520.10009817936442453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG350.001441064238.89584719
CGAAACC706.9852304E-637.94895669
GTATCAA39000.037.717161
GTGGTAT4100.034.8807071
TATCAAC43000.034.0284232
ATCAACG44650.032.910193
TCAACGC45250.032.545084
AACGCAG45700.032.154056
CACTAGT4050.032.1038470
CGTAATG2356.730261E-1132.03193770
CAACGCA46150.031.9142235
AATCGGT450.00428169631.15089651
ACGCAGA49100.029.996777
CGCAGAG49550.029.7243488
AGAGTAC50100.029.46592711
CAGAGTA51250.028.9374810
GCAGAGT51500.028.5988649
TAGTCAC1653.6379788E-1227.0964238
GTCGAGG2805.14774E-1026.88394570
CCGAAAC759.6293975E-426.058868
Produced by FastQC (version 0.11.5)