FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908816
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37960.41768630833964193No Hit
GTGTACAACCTGAGGATTCTGCAACTTACTACTGTCAACAGAGTTACCATATCCCCCGCACTTTCGGCGGAGGGA33560.36927166775232834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32480.35738807415362406No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28090.3090834668403725No Hit
ACCATATCCCCCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA27380.3012711043819651No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24080.2649601239414799No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24000.2640798577489833No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23150.2547270294537068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22270.24504410133624407No Hit
GTAGGAGACAGAGTCACCATCCCTTGCCGGGCAAGTCAGGGCATTGGCTCCTATTTAAATTGGTATCAACAAAAA21060.23173007517473285No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20780.22864914350099472No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20640.22710867766412562No Hit
GAGTTACCATATCCCCCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19720.2169856164504146No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19230.21159398602137286No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19110.21027358673262794No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18580.2044418232073379No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17860.1965194274748684No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT17770.19552912800830968No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17540.19299836270488194No Hit
GGATATGGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGTACACTGCTGATGGTGAGAGCGAAAT17510.19266826288269573No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17090.1880468653720885No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16370.18012446963961903No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16290.1792442034471224No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG15790.1737425397440186No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15750.17330240664777027No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15220.16747064312248025No Hit
ACTTACTACTGTCAACAGAGTTACCATATCCCCCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA14980.16482984454499042No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14590.16053854685656943No Hit
GGTTGTACACTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACT14400.15844791464938995No Hit
AGTCAGGGCATTGGCTCCTATTTAAATTGGTATCAACAAAAACCAGGGAA14390.1583378813753279No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14070.15481681660534144No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13990.15393655041284485No Hit
TGGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGTACACTGCTGATGGTGAGAGCGAAATCTGTC13500.14854491998380312No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13330.1466743543247478No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13030.1433733561028855No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12960.14260312318445098No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12620.13886199186634038No Hit
GCAGTGTACAACCTGAGGATTCTGCAACTTACTACTGTCAACAGAGTTAC12370.13611116001478846No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCCCTTGCCGGGCAAGTCAGGGCATTGGCTC12180.13402052780760904No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12130.13347036143729862No Hit
GTAGTAAGTTGCAGAATCCTCAGGTTGTACACTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCA12120.13336032816323656No Hit
CTCCTGATCCATGGTGCTTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTT12050.13259009524480203No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12050.13259009524480203No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCCCTTGCCG11680.1285188641045052No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11450.12598809880107745No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCCCTTGCCGGGCAAGTCAGG11330.12466769951233253No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCTTCC11010.12114663474234609No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10910.12004630200172532No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10720.11795566979454589No Hit
CCCCTAAACTCCTGATCCATGGTGCTTCCAGTTTGCAGAGTGGGGTCCCA10710.1178456365204838No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10580.1164152039576768No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10350.11388443865424905No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10300.11333427228393868No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAATAGGAGCCA10120.1113536733508213No Hit
CACCATCAGCAGTGTACAACCTGAGGATTCTGCAACTTACTACTGTCAACAGAGTTACCATATCCCCCGCACTTT9960.10959314096582806No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9940.10937307441770393No Hit
GTTGTACACTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACT9920.10915300786957975No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAAT9870.10860284149926938No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9800.10783260858083484No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9750.10728244221052446No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9600.10563194309959331No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9470.10420151053678632No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9440.10387141071460008No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9440.10387141071460008No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9280.10211087832960689No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9280.10211087832960689No Hit
GTCACCATCCCTTGCCGGGCAAGTCAGGGCATTGGCTCCTATTTAAATTGGTATCAACAAAAACCAGGGAAAGCC9250.10178077850742065No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9110.10024031267055157No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9100.10013027939648951No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9100.10013027939648951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT554.3655746E-1156.20229739
ATCGTAT507.192102E-848.08418339
TATCGTA507.3298E-847.96843738
TCGTATG4550.046.8930440
CTAATCG1100.046.39266634
CGTATGC4700.045.4808441
ATCTCGT4650.044.86450237
ATGCCGT4500.044.02353744
CTTATAC7900.043.0717961
GCGTATG803.45608E-1143.016840
CCGTCTT4250.042.8356447
GCTAATC6450.042.67459533
TTAACGG405.3759446E-542.6000235
TAATCGC1050.042.19430535
GTATGCC5250.042.1459442
TTATACA8250.041.6505782
TCGCGTA759.331416E-1041.11580338
CCCACGA7950.041.09224719
ACGAGAC8150.040.9260922
ATCTCCG8350.040.73262810
Produced by FastQC (version 0.11.5)