FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908816
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66800.7350222707346702No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37590.4136150771993451No Hit
GTGTACAACCTGAGGATTCTGCAACTTACTACTGTCAACAGAGTTACCATATCCCCCGCACTTTCGGCGGAGGGA34560.3802749951585359No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27480.3023714371225859No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26140.2876269783982676No Hit
ACCATATCCCCCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA24070.2648500906674178No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23750.26132902589743134No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22870.25164609777996866No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22230.24460396823999578No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21630.2380019717962712No Hit
GAGTTACCATATCCCCCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT20740.2282090104047464No Hit
GTAGGAGACAGAGTCACCATCCCTTGCCGGGCAAGTCAGGGCATTGGCTCCTATTTAAATTGGTATCAACAAAAA18740.20620235559233113No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18630.20499198957764828No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17440.1918980299642612No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17170.18892713156458513No Hit
GGATATGGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGTACACTGCTGATGGTGAGAGCGAAAT17030.18738666572771606No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16780.18463583387616417No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15980.17583317195119805No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15810.17396260629214275No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15610.1717619408109012No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15440.16989137515184594No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15320.16857097586310102No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15290.16824087604091478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15020.1652699776412387No Hit
GGTTGTACACTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACT14970.16471981127092833No Hit
ACTTACTACTGTCAACAGAGTTACCATATCCCCCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA14960.16460977799686627No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14650.16119874650094188No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14390.1583378813753279No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14010.154156616960969No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13840.1522860513019137No Hit
AGTCAGGGCATTGGCTCCTATTTAAATTGGTATCAACAAAAACCAGGGAA13560.14920511962817556No Hit
CTCCTGATCCATGGTGCTTCCAGTTTGCAGAGTGGGGTCCCATCAAGGTT13490.14843488670974103No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13470.14821482016161688No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13370.14711448742099612No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12660.1393021249625887No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12550.13809175894790585No Hit
TGGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCCTCAGGTTGTACACTGCTGATGGTGAGAGCGAAATCTGTC12410.13655129311103678No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCCCTTGCCGGGCAAGTCAGG11880.13071952958574673No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11640.1280787310082569No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11440.12587806552701536No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCCCTTGCCGGGCAAGTCAGGGCATTGGCTC11380.12521786588264291No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11380.12521786588264291No Hit
GTAGTAAGTTGCAGAATCCTCAGGTTGTACACTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCA11260.12389746659389798No Hit
GCAGTGTACAACCTGAGGATTCTGCAACTTACTACTGTCAACAGAGTTAC10980.12081653492015985No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10920.1201563352757874No Hit
GTTGTACACTGCTGATGGTGAGAGCGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACT10860.11949613563141494No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10830.11916603580922872No Hit
CCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCCCTTGCCG10790.1187259027129804No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCTTCC10790.1187259027129804No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10530.11586503758736642No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10330.1136643721061249No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10260.11289413918769035No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10170.11190383972113167No Hit
GTTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAATAGGAGCCA10080.11091354025457298No Hit
CCCCTAAACTCCTGATCCATGGTGCTTCCAGTTTGCAGAGTGGGGTCCCA10050.11058344043238676No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10020.11025334061020052No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCGCT9930.10926304114364184No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9820.10805267512895901No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9790.10772257530677277No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9540.10497174345522085No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9530.10486171018115878No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTTAAAT9340.10277107797397934No Hit
CACCATCAGCAGTGTACAACCTGAGGATTCTGCAACTTACTACTGTCAACAGAGTTACCATATCCCCCGCACTTT9310.10244097815179311No Hit
GTCACCATCCCTTGCCGGGCAAGTCAGGGCATTGGCTCCTATTTAAATTGGTATCAACAAAAACCAGGGAAAGCC9210.10134064541117234No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9110.10024031267055157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)