FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215324
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGTAGAAAGA43350.3566950047888464No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31480.25902557671863635No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCCAGAGAGTGTCGCTGGTTGTAAATGAG30100.24767058002639625No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28470.2342585187159967No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27650.22751134676843374No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24290.19986439830037092No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCCAGAGAGTGTCG24150.19871244211420164No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23400.19254124825972332No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCGTCTCCTGGACAGTCGATCACCATCTCCTG23270.19147157465828044No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA22780.18743972800668793No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22100.18184451224529427No Hit
GTCTATTACTGTGTAAGAAGACGCAGTGGCTGGTACGCTGACCACTGGGGCCAGGGAACCCTGGTCACCATCTCC21750.17896462177987105No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT19930.16398919135967033No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18770.15444441153141056No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18650.15345702051469404No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18500.15222278174379836No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC18430.1516468036507137No Hit
TCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTACTGCAGCTCATTT18320.15074169521872358No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17980.14794408733802672No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17920.14745039182966846No Hit
GATTATTACTGCAGCTCATTTACAACCAGCGACACTCTCTGGGTGTTCGG17620.14498191428787716No Hit
CCCAGAGAGTGTCGCTGGTTGTAAATGAGCTGCAGTAATAATCAGCCTCG16970.1396335462806626No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTAAGCAACTATAACATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGA16930.1393044159417571No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTAATAATTATGTGTCCTGGTACCAGCAGCACCCAGGCAAAGC16090.1323926788247414No Hit
CTCTCTGGGTGTTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCC16030.1318989833163831No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15550.127949419249517No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15540.1278671366647906No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15430.12696202823280048No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15420.12687974564807408No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC15030.12367072484374536No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTGTTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT14960.12309474675066072No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14700.12095539954777493No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC14570.119885725946332No Hit
CCCTGGGGAGATGGTGACCAGGGTTCCCTGGCCCCAGTGGTCAGCGTACC14370.11824007425180445No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG13730.11297398882931631No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13620.11206888039732615No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCCAGAGAGTGTCGC13450.11067007645697773No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTAATAATTATGTGTCCTGGTACCAGCAGCA13430.11050551128752498No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13390.11017638094861947No Hit
GTCTCTGGAGACGGTGAATCGGCCCTTCACAGAGTCTGTGTAAAATATGG13320.10960040285553482No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13020.10713192531374349No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13000.10696736014429073No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12920.10630909946647972No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12670.1042520348483203No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12420.10219497023016084No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12360.10170127472180258No Hit
TTCTTACACAGTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGTAGAAAGAGAGAGTTCTTGACA12250.10079616628981243No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCGTCTCCTGGACAGTCGATCAC12240.10071388370508606No Hit
GTGCTGGGTTTTCCTTGTTGCTGTTTTAGAAGGTGTCCAGTGTGAGGTGC12200.10038475336618055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1200.051.7361340
ACCGTAC1350.045.46484427
CGTATGC1300.045.163541
GGTATCA10500.044.4558071
TCTGGAG17350.043.89268570
GGATGGG10850.043.6302870
GACCGTA1450.042.3153926
GTACTAG1500.040.9977330
CGTACTA1600.040.53386729
ATCTCGT1200.040.09387637
CCTAACG350.001433726438.9361842
GCCGTCT1450.038.36889346
GTATCAA28650.038.2937971
GTGGTAT3000.036.3434331
ACGAGAC1800.035.95597522
TCTCGTA1350.035.68195738
ACTAGAT1550.035.32810232
CACGAGA1850.034.9841921
TATCAAC31750.034.8739472
ATGCCGT1600.034.6771344
Produced by FastQC (version 0.11.5)