FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215324
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGTAGAAAGA40530.33349131589600794No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30140.24799971036530175No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29570.24330960303589824No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCCAGAGAGTGTCGCTGGTTGTAAATGAG26980.22199841359176647No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA25520.20998515622171537No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24110.19838331177529614No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG23300.19171842241245957No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23260.19138929207355404No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCGTCTCCTGGACAGTCGATCACCATCTCCTG22600.18595864148161315No Hit
GTCTATTACTGTGTAAGAAGACGCAGTGGCTGGTACGCTGACCACTGGGGCCAGGGAACCCTGGTCACCATCTCC22240.18299646843146355No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21560.1774012526700699No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACACCCAGAGAGTGTCG20520.1688438638585266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19940.16407147394439672No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19570.16102701830952076No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT18920.1556786503023062No Hit
TCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTACTGCAGCTCATTT18630.15329245534524127No Hit
GATTATTACTGCAGCTCATTTACAACCAGCGACACTCTCTGGGTGTTCGG17420.14333626259334958No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17330.1425957193308122No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTAAGCAACTATAACATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGA16970.1396335462806626No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC16750.1378233294166823No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16720.13757648166250316No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16690.13732963390832403No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16390.1348611563665327No Hit
CTCTCTGGGTGTTCGGCGGAGGGACCACGCTGACCGTCCTAGGTCAGCCC16340.1344497434429008No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16030.1318989833163831No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTACTAATAATTATGTGTCCTGGTACCAGCAGCACCCAGGCAAAGC15860.1305001793760347No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTGTTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT15860.1305001793760347No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15720.1293482231898654No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC15550.127949419249517No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15040.12375300742847174No Hit
CCCAGAGAGTGTCGCTGGTTGTAAATGAGCTGCAGTAATAATCAGCCTCG14890.12251876865757609No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14630.12037942145469029No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCATGTCTGCGTCTCCTGGACAGTCGATCAC14420.11865148717543635No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14400.11848692200598358No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTACTAATAATTATGTGTCCTGGTACCAGCAGCA14020.11536018378638124No Hit
GTGCTGGGTTTTCCTTGTTGCTGTTTTAGAAGGTGTCCAGTGTGAGGTGC13960.11486648827802298No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13490.11099920679588324No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13410.11034094611807221No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13330.1096826854402612No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13010.10704964272901711No Hit
GTCTCTGGAGACGGTGAATCGGCCCTTCACAGAGTCTGTGTAAAATATGG12620.10384062192468839No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12500.10285323090797185No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12400.1020304050607081No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC12310.10128986179817069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC401.3274985E-859.580843
GGATGGG10450.051.71412770
TAACGCC507.691233E-847.6784064
CCTAACG507.708695E-847.6646732
GGTATCA10400.045.212031
TCTGGAG16850.042.90145570
TCCGAGA7150.041.2266470
GTATCAA27250.038.2616041
TATCAAC30900.033.7156452
ATCAACG31150.033.1171653
TCAACGC31250.032.9117244
GTGGTAT2800.032.855951
CAACGCA31750.032.394765
AGGATGG10900.032.2966669
AACGCAG32100.032.2537426
TTCGATC751.6997947E-632.12546539
GTGAGCG4500.031.19264470
TCTCCGA8400.030.7927368
AGAGTAC33550.030.75698711
CGCAGAG33600.030.7124848
Produced by FastQC (version 0.11.5)