FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056879
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37110.3511281802363374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36010.340720177049596No Hit
CAATATGACAGTCGTTTTTCGTGGACGTTCGGCCAAGGGACCAAGGTGGA29380.2779883032967823No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA28630.2708919374876405No Hit
GTCATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC27120.2566045876585683No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25660.24279032888343885No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC24590.23266618032906322No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24160.22859759726515522No Hit
GATCTATACGGCATCTAGTTTAGAAAGAGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA22840.21610799344106563No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20500.19396733211654313No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20290.19198034968998343No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19360.18318085608664758No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19090.1806261643953565No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18850.17835532733643114No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCG18660.17655758133144853No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18090.17116434331650074No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17930.1696504519438838No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17810.16851503341442114No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17620.16671728740943853No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17460.1652033960368216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17140.16217561329158778No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16890.15981015802187382No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGA16520.15630928422269721No Hit
GAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTATACGGCAT16490.15602542959033153No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15920.15063219157538374No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15450.14618513566832153No Hit
GTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAA15360.14533357177122452No Hit
GTGTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT15300.14476586250649318No Hit
GTCAGAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTATACGG15050.14240040723677924No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14780.13984571554548816No Hit
GTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC14430.13653407816788865No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14300.1353040414276374No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14250.13483095037369464No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13890.13142469478530655No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13590.1285861484616498No Hit
ACTTATTACTGCCAACAATATGACAGTCGTTTTTCGTGGACGTTCGGCCA13420.12697763887824434No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13380.1265991660350901No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT13230.12517989287326176No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13170.12461218360853041No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC12960.12262520118197068No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12820.12130054623093088No Hit
GTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAA12680.11997589127989107No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12580.1190297091720055No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12450.11779967243175425No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12350.11685349032386867No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCT12080.11429879863257761No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11860.11221719799522935No Hit
GTATTACTGTGCAAGAGACCCATCCCTCGGGGCCTTCCGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC11780.11146025230892088No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11630.11004097914709252No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11540.10918941524999551No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTT11540.10918941524999551No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11440.10824323314210993No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11190.105877777872396No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACC11190.105877777872396No Hit
CCTACTAACACTCTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA11160.10559392324003031No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC10570.1000114488035054No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG10570.1000114488035054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG200.005649463755.32656567
TCGTATG1750.043.3713740
ACGAGAC2600.043.2765322
GAGCCTA2400.042.80517631
ATCTCGT1700.042.5076737
CGTATGC1950.040.75274741
ACCGGAG2650.039.91527
CCGTCGT350.0012833539.8232647
GACCGGA2700.039.16655726
GTATCAA30150.039.0142361
AGACCGG2900.037.63815725
CTTATAC2950.036.9844631
TATACAC3000.036.364613
GAGACCG3050.035.78201324
ATCTCCG3250.035.6602310
TCTCCGA3450.035.5673411
AGCCTAT2700.035.54623432
ATCAACG32950.035.385143
CGAGACC3200.035.16551223
TCAACGC33450.034.8578644
Produced by FastQC (version 0.11.5)