FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1056879
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39530.37402578724716834No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32070.3034406019989043No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA30050.28432772341961565No Hit
GTCATATTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC26780.2533875684917573No Hit
CAATATGACAGTCGTTTTTCGTGGACGTTCGGCCAAGGGACCAAGGTGGA26290.24875127616311804No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24540.23219308927512045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23880.2259482873630756No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23180.21932501260787657No Hit
GATCTATACGGCATCTAGTTTAGAAAGAGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAGTTCA23060.2181895940784139No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22420.21213402858794622No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21570.2040914806709188No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20510.1940619503273317No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19750.1868709663074013No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19030.18005845513062518No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCT18580.1758006356451401No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17600.16652805098786141No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17590.16643343277707287No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17350.1641625957181475No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16760.1585801212816226No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16460.15574157495796587No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16320.15441692000692606No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16310.1543223017961375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16000.15138913726169223No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15890.15034833694301808No Hit
GAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTATACGGCAT15510.14675284493305288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15330.14504971713885884No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCG15270.14448200787412752No Hit
GTCTGCATCTGTAGGAGACACAGTCACCATCACTTGCCGGGCCAGTCAGA15260.14438738966333894No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14990.1418326979720479No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14800.1400349519670653No Hit
GTATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC14320.13549327784921453No Hit
GTGTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT13820.13076236730978663No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13790.13047851267742097No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13760.1301946580450553No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13390.12669378424587865No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13320.12603145677035876No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13070.12366600150064483No Hit
ACTTATTACTGCCAACAATATGACAGTCGTTTTTCGTGGACGTTCGGCCA13070.12366600150064483No Hit
ATCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATT12960.12262520118197068No Hit
GTCAGAGTGTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTATACGG12800.12111130980935376No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT12490.11817814527490848No Hit
GTTAGTAGGTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAA12380.11713734495623433No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11960.11316338010311494No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11790.11155487051970944No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11730.1109871612549781No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA11730.1109871612549781No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11680.11051407020103532No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCTCTTTCTAAACT11520.10900017882841839No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11420.10805399672053281No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11390.10777014208816714No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11280.106729341769493No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11260.10654010534791589No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC10980.10389079544583628No Hit
GTATTACTGTGCAAGAGACCCATCCCTCGGGGCCTTCCGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC10960.10370155902425916No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10950.1036069408134706No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10890.10303923154873927No Hit
CCTACTAACACTCTGACTGGCCCGGCAAGTGATGGTGACTGTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA10690.1011468673329681No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10630.10057915806823677No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGC251.967185E-458.2545766
CTATCGG200.00609960154.2648765
CCTAACG405.4061646E-542.560432
GTGGTAT2950.040.415471
GTACGAA459.723326E-538.52620343
CTAACGC451.0838188E-437.8225443
GTATCAA28150.034.4880071
GACAGGT2200.032.5160831
TACGTAT1303.1146265E-530.5010370
TATCAAC31200.030.4470792
ATCAACG32050.029.4201563
TCAACGC32600.028.9251754
CAACGCA33000.028.6804355
AACGCAG33850.028.1614026
AATGGTA6300.027.27340970
GTACGTA1306.254304E-726.91096569
GCCTGAC3300.026.8234317
TACGAAA658.338113E-426.7093244
ACGCAGA36050.026.4428127
CGCAGAG36100.026.4061898
Produced by FastQC (version 0.11.5)