FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878846
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGCAAC90830.4834350447029719No Hit
CCCCTATACTTGTCGTCTATGGTAAGAACAAGCGCCCCTCAGGGATCCCA83100.4422927690720793No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC82480.4389928711560181No Hit
GTATAGGGGCCTGTCCGGGCTTCTGTTGGTACCAACTTGGATAATAGTTT72190.3842252105813888No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC54720.2912426031723728No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42460.22598978309025858No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAAGGCAACATGGCCGGCA39380.20959674182982535No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG36610.19485364952742268No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33830.18005733306508356No Hit
ACTCAGGACCCTGCTGTGTCTGTGCCCTTGGGGCAGACTGTCATTATCACATGCCAAGGAGACAGTCTCAGAAA32330.17207370907461284No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA32050.1705834325963916No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31180.16595293068191858No Hit
GTGTGGCGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30450.16206756700655614No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT30340.16148210124725498No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29620.15764996173182902No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC28650.15248721821799124No Hit
GTCTGTGCCCTTGGGGCAGACTGTCATTATCACATGCCAAGGAGACAGTCTCAGAAACTATTATCCAAGTTGGTA28380.15105016589970652No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGCCCTTGGGG27830.1481228371032006No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27640.1471115780644076No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGTCTGTGCCCTTGGGGCAGA27570.1467390089448523No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27010.14375845598840992No Hit
GTTTGGAGGGTGTGGCGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26980.1435987835086005No Hit
GTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC26980.1435987835086005No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26650.14184238623069695No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG26520.1411504721515228No Hit
GTCATTATCACATGCCAAGGAGACAGTCTCAGAAACTATTATCCAAGTTGGTACCAACAGAAGCCCGGACAGGCC26230.1396069715133651No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25980.1382763675149533No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25620.13636029775724035No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC25490.13566838367806622No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25140.13380553808028972No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA25020.13316684816105206No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24710.13151689920302143No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24590.13087820928378377No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG24560.13071853680397436No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23980.127631535527659No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGCGGTCTCCA23720.12624770736931074No Hit
CTATTATCCAAGTTGGTACCAACAGAAGCCCGGACAGGCCCCTATACTTGTCGTCTATGGTAAGAACAAGCGCCC23530.12523644833051778No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23460.12486387921096247No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG22750.12108496385547299No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22740.12103173969553652No Hit
CTACTATTGTAACTCTCGGGACATCGTTGCCGGCCATGTTGCCTTCGGCG22220.11826408337883998No Hit
CTATTGTAACTCTCGGGACATCGTTGCCGGCCATGTTGCCTTCGGCGGAG22090.11757216929966585No Hit
GTCCCAGTGATGGTCAAGGAAGCTGTATTTCCTGAGGTGGAGCCAGAGAATCGGTCTGGGATCCCTGAGGGGCG21710.11554965122207994No Hit
CCTTGGGGCTGGTCGGGGATGCTGACGAGACGGCGACCAGGGTTCCCTGGCCCCAAATGTCATAGTTATGTCGA21460.11421904722366813No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21440.1141125989037952No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21350.11363358146436696No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC21330.113527133144494No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21150.11256909826563753No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21110.11235620162589163No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGCGGTCTCCACTCCCGCCTTGA20970.11161106338678102No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20850.11097237346754338No Hit
CTGTATTTCTGTGCGAGAGGAGACTATTACTATGAGAGTCGACATAACTA20460.10889663123002098No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20240.10772569971141861No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGC20010.10650154403287976No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19910.10596930243351504No Hit
GTATTTCTGTGCGAGAGGAGACTATTACTATGAGAGTCGACATAACTATGACATTTGGGGCCAGGGAACCCTGGT19910.10596930243351504No Hit
GTTACAATAGTAGTCAGCCTCATCTTCCGCCTGAGTCCCAGTGATGGTCA19800.10538383667421385No Hit
GAAATACAGAGCCGTGTCCGCGGCGGTCACAGCGGCCAGCTTCAGGGAAA19750.1051177158745315No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19450.10352099107643735No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC19430.1034145427565644No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19370.10309519779694556No Hit
GTCCTGTCCCAGGTGCAACTACACCAATGGGGCGCAGGAATTTTGAGGCC19270.10256295619758085No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18840.10027431732031258No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18790.10000819652063021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)