FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925356
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41840.4521503075573077No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36970.399521913728338No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG29370.3173913607303568No Hit
GTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTT27260.2945893256217067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26670.28821340111265287No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTC22980.2483368563017909No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22620.24444646168609702No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21760.23515274121527283No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21100.22802035108650076No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20630.22294122478267822No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20510.22164442657744693No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19880.21483623599998272No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATATTGGGATTTATTACTGCATGCAATCTCTACAAATT19750.2134313712776488No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19550.2112700409355967No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTT18970.20500218294364547No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18960.20489411642654287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18560.20057145574243856No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18500.19992305663982293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17950.19397939819917956No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTTCTAATCGGGCC17710.19138580178871697No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17690.19116966875451177No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17430.188359939309844No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGTCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAAGTA16640.17982268445873806No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGA16240.17550002377463375No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15980.17269029432996597No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT15850.1712854296076321No Hit
GTAATAGACGGCCGTGTCAGCGGCGGTCACAGAGTTCAGGTTCAGGGAGA15140.16361270689334698No Hit
GTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTTCTAATCGGGCCTCCGGGGTCCCTGA15140.16361270689334698No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC15130.16350464037624438No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG15070.16285624127362874No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14780.15972231227765313No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG14630.15810131452111403No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGACTCAGTCTCCACTCTCC14570.15745291541849837No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14370.15529158507644625No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14310.15464318597383062No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13770.14880759405028984No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13570.1466462637082377No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13200.14264780257544124No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13170.1423236030241334No Hit
CCTTGGTCCCTTGGCCGAACGTTCGAGGAATTTGTAGAGATTGCATGCAGTAATAAATCCCAATATCCTCAGCCT13110.14167520392151775No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12720.1374606097545161No Hit
GTCTCTGGTGGCTCCATGAGTAGTTACTACTGGAGTTGGATCCGGCAGCC12620.13637994458349No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12540.13551541244666918No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12540.13551541244666918No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12530.13540734592956657No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGACTCAGTCTCCACT12420.13421861424143788No Hit
GTAATAAATCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12300.13292181603620662No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12240.13227341693359096No Hit
CTACAAATTCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACG12050.13022015310864143No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11980.12946368748892317No Hit
GCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGC11680.12622169197584499No Hit
TTATTACTGCATGCAATCTCTACAAATTCCTCGAACGTTCGGCCAAGGGA11370.12287162994566415No Hit
CAGTAATAAATCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11340.12254743039435632No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11290.12200709780884329No Hit
GAGTGGAGGCTGAGGATATTGGGATTTATTACTGCATGCAATCTCTACAAATTCCTCGAACGTTCGGCCAAGGGA11170.12071029960361201No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC11110.12006190050099638No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11050.11941350139838074No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCACCACAATATCC11010.1189812353299703No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10970.11854896926155989No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAG10880.11757637060763641No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10830.11703603802212337No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA10800.11671183847081555No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10800.11671183847081555No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT10630.11487470768007124No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10600.11455050812876341No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10600.11455050812876341No Hit
GGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTTCTAAT10590.11444244161166083No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10430.1127133773380191No Hit
GCCCTGGCTTCTGCAAGTACCATTCCAAATAGTTGTATCCATTACTATGC10430.1127133773380191No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10350.11184884520119825No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC10250.11076818003017216No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATATTGGGATTTATTACTGCATGCAATCTCTA10240.11066011351306958No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10220.11044398047886435No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10180.11001171441045392No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10170.10990364789335132No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9960.10763425103419656No Hit
GAATTTGTAGAGATTGCATGCAGTAATAAATCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT9920.10720198496578615No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9890.10687778541447833No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9780.10568905372634965No Hit
GTGGAGGCTGAGGATATTGGGATTTATTACTGCATGCAATCTCTACAAAT9670.10450032203822097No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9650.10428418900401576No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9360.10115026000804014No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9330.10082606045673233No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA9310.10060992742252711No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9290.10039379438832191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)