FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925356
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42010.453987438348052No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33790.36515676128970903No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG26060.28162134356939383No Hit
GTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTT24600.26584363207241324No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23680.25590151249897336No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23510.2540643817082291No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23350.25233531743458737No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22850.24693199157945697No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTC22510.24325772999796835No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21350.23072201401406595No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20720.22391382343660168No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTT19070.20608284811467156No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18960.20489411642654287No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18750.20262471956738812No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATATTGGGATTTATTACTGCATGCAATCTCTACAAATT18620.20121985484505422No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18190.19657299460964212No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18170.19635686157543691No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18150.1961407285412317No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17920.19365519864787173No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG16240.17550002377463375No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16150.17452742512071032No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16070.17366289298388946No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15820.17096123005632427No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15700.16966443185109298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15660.16923216578268258No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTTCTAATCGGGCC15410.16653050285511736No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15390.1663143698209122No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGTCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAAGTA15190.16415303947886004No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGA15130.16350464037624438No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT14540.15712871586719057No Hit
GTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTTCTAATCGGGCCTCCGGGGTCCCTGA14440.1560480506961645No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGACTCAGTCTCCACTCTCC14430.15593998417906188No Hit
GTAATAGACGGCCGTGTCAGCGGCGGTCACAGAGTTCAGGTTCAGGGAGA14400.15561578462775408No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14370.15529158507644625No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13990.1511850574265472No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13700.14805112843057158No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG13570.1466462637082377No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG13320.1439446007806725No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGGTGACTCAGTCTCCACT13300.1437284677464673No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13160.1422155365070308No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12890.13929774054526042No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12760.13789287582292653No Hit
CCTTGGTCCCTTGGCCGAACGTTCGAGGAATTTGTAGAGATTGCATGCAGTAATAAATCCCAATATCCTCAGCCT12590.1360557450321822No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12410.13411054772433528No Hit
GTAATAAATCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA12300.13292181603620662No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA12270.1325976164848988No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12230.13216535041648836No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11960.12924755445471797No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11940.12903142142051274No Hit
GAGTGGAGGCTGAGGATATTGGGATTTATTACTGCATGCAATCTCTACAAATTCCTCGAACGTTCGGCCAAGGGA11860.12816688928369188No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11790.12741042366397365No Hit
CTACAAATTCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACG11550.12481682725351108No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11510.12438456118510065No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11220.12125063218912506No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAG11170.12071029960361201No Hit
CAGTAATAAATCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA11110.12006190050099638No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11080.11973770094968857No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11040.11930543488127812No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA10910.11790057015894423No Hit
CTCCTGCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT10810.11681990498791817No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10770.11638763891950774No Hit
GTGGAGGCTGAGGATATTGGGATTTATTACTGCATGCAATCTCTACAAAT10610.11465857464586601No Hit
TTATTACTGCATGCAATCTCTACAAATTCCTCGAACGTTCGGCCAAGGGA10480.11325370992353215No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10480.11325370992353215No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10360.11195691171830084No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT10330.11163271216699303No Hit
GAATTTGTAGAGATTGCATGCAGTAATAAATCCCAATATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT10300.11130851261568521No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCACCACAATATCC10250.11076818003017216No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10230.11055204699596695No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10230.11055204699596695No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC10220.11044398047886435No Hit
GCATAGTAATGGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGC10160.10979558137624872No Hit
GTCTCTGGTGGCTCCATGAGTAGTTACTACTGGAGTTGGATCCGGCAGCC10100.10914718227363306No Hit
GCCCTGGCTTCTGCAAGTACCATTCCAAATAGTTGTATCCATTACTATGC10060.10871491620522264No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9990.10795845058550438No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9970.10774231755129918No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9960.10763425103419656No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGAT9910.10709391844868352No Hit
GGATACAACTATTTGGAATGGTACTTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGACTTCTAAT9760.10547292069214442No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9730.1051487211408366No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC9620.10395998945270793No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9450.10212285866196362No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9310.10060992742252711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGC150.002495796767.999793
GCCGTTG200.006773927752.8447255
GTCTATC200.006828095852.73840354
CCATACG200.007789212351.0108642
ATAAGCG200.00779920450.99432412
TGGTATC2150.045.870232
GGTATCA8300.045.4918631
GTGGTAT2250.043.843411
GTTATTA1158.10693E-637.12745370
TACTGAT5800.036.80739270
ACGAACC501.6972199E-435.04534552
GTATCAA20450.034.7649651
GATTCAC1650.030.9240341
ACGGGTT450.00494214130.24991617
CGATGTT450.004968344230.21722614
AATAGCG604.3488684E-429.85299762
TATCAAC23850.029.3731332
ATCAACG24600.028.4714553
TCAACGC24650.028.4121674
CTGTAAC500.007260089727.95995950
Produced by FastQC (version 0.11.5)