FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130409
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGATAGCTCACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA54610.48309947992275365No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43260.38269334373664754No Hit
CAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA36630.3240420060349838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31380.2775986390766528No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27770.2456632953205433No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27610.24424787842276555No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26970.23858621083165474No Hit
GTGTATTACTGTCAGCAGTATGATAGCTCACCTCCGTGGACGTTCGGCCA24620.21779727514554467No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23710.20974709153943394No Hit
GTATGATAGCTCACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23630.2090393830905451No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGCAGATT23490.2078008933049896No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22570.19966224614276779No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACC20990.18568500427721293No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20700.18311956114999084No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20640.1825887798133242No Hit
CATCTATGGTGCATCCGGCAGGGCCGCTGGCATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCA20000.17692711222221336No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19270.1704692726261026No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19230.17011541840165817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19120.169142319284436No Hit
GTCCCAGACCCACTGGCACTGAACCTGTCTGGGATGCCAGCGGCCCTGCC18840.166665339713325No Hit
GTAATACACTGCAAAATCTGCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA18080.15994210944888088No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG17890.15826130188276985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17700.15658049431665882No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17540.15516507741888114No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17500.15481122319443671No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGGTGAGCTATCATACTG17230.15242270717943682No Hit
CCATAGATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA16620.14702643025665932No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16170.1430455702316595No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15630.13826853820165974No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15560.137649293308882No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15490.13703004841610425No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGCAGATTTTGCAGTGTATTACTGTCAGCAGTATGATAGCTCA14980.13251840705443782No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14710.13012989103943795No Hit
GTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGGTGCAT14200.1256182496777715No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14110.12482207767277154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14100.12473361411666044No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13990.12376051499943824No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGCCTGGTACCAGCAGAAACCTGGC13770.12181431676499392No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT13050.11544494072499423No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12890.11402952382721651No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGTCTGGGATGCCA12890.11402952382721651No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12740.11270257048554994No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12370.10942941890943897No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12320.10898710112888343No Hit
ATCATACTGCTGACAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGT12250.10836785623610569No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12040.10651012155777244No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11990.1060678037772169No Hit
CTATCATACTGCTGACAGTAATACACTGCAAAATCTGCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC11970.1058908766649947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAA200.006564170653.26746458
GGTATCA11900.048.3037151
CGTATGC950.043.72564341
TCGTATG955.456968E-1240.00797740
GTATCAA34500.036.575951
TCTCCGA2950.035.73106811
GTGTACG551.6188686E-435.32869368
CCCACGA3000.034.0036519
ACGCAAC400.002788104134.00214814
GGTCCAT800.002995958333.50420870
ACGAGAC3050.033.4565722
TATCAAC37300.033.186122
GCCGTCT1400.032.3621546
ATCTCCG3300.031.9414110
ATCAACG38850.031.5994973
TCAACGC39250.031.277464
CAACGCA40050.030.7376045
AACGCAG40550.030.5249716
GATTCAC2150.030.0577141
CACGAGA3400.030.01251421
Produced by FastQC (version 0.11.5)