FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146582
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43420.37869075216600295No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39800.34711865352848725No Hit
GAGTTACATTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCAT28190.2458611769589964No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28050.24464015656970023No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24740.21577174593705464No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22690.1978925188080748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22560.1967587141608712No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22190.19353173170344556No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21820.19030474924601992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21800.19013031776183473No Hit
GACTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTTAC21560.1880371399516127No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGCCAAGCCAGACCATTGGTCCTTTTTTGAATTGGTTTCAGCAGAAA20370.17765846664259513No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19320.16850081372287373No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19300.16832638223868857No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAAGTGACATCCAGATGACCCAGTC18280.15943037654524492No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18090.1577732774454858No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17800.15524402092480086No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGGGTGAAGTCTGTC17420.15192982272528263No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17280.15070880233598644No Hit
GTGCAGCCACAGTTCGCTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC17110.1492261347204125No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17040.1486156245257644No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16730.14591193652089426No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16530.14416762167904257No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16400.14303381703183898No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16050.1399812660585985No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15970.13928354012185784No Hit
ATCTGGGACAGACTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATT15180.13239349649654364No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14830.12934094552330316No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14800.1290792982970254No Hit
ACTTACTACTGTCAACAGAGTTACATTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAA14700.12820714087609955No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAATTCCACCTTGGTCC14650.12777106216563663No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGTGACAT14470.12620117880797013No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14130.12323584357682225No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13880.12105545002450763No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13870.12096823428241504No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13850.12079380279822988No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13770.12009607686148918No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13100.11425262214128601No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGCCAAGCCAGA12700.11076399245758262No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12500.10901967761573093No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12330.10753701000015699No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11750.10247849695878707No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGGGTGAAGTCTGTCCCAGATTCCCCGCCA11660.10169355527995382No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11640.10151912379576865No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11590.10108304508530573No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11560.10082139785902795No Hit
GGGTGAAGTCTGTCCCAGATTCCCCGCCACTGAACCTTGATGGGACCCCA11510.10038531914856504No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC11470.10003645618019472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC950.058.08755541
ATACCGT252.7941217E-454.261386
GAACGAT200.007173396652.08539645
CGCGTAT200.00747831551.53991339
TCGTATG850.048.59834740
GGTATCA13450.043.8730471
ATGCCGT1100.040.98024744
ATCTCCG1750.040.69070410
GTATCAA35300.037.9483761
GTGGTAT3300.035.968711
TATACTG853.5106495E-935.9082645
TCTCCGA2100.035.52363211
GCGTATG601.3630017E-534.42383240
ATCGCGT400.002687301534.25851437
ACGAGAC2000.033.92223722
CTTATAC2300.032.4388661
TAAGACA2000.032.35173831
CCCTAAG2100.032.34781628
TATCAAC41600.031.9568212
TAACGTA652.3568253E-531.77584540
Produced by FastQC (version 0.11.5)