FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146582
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41250.35976493613191207No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34920.30455737138730593No Hit
GAGTTACATTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAACTGTGGCTGCACCAT30310.26435091428262436No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29790.25981569569380997No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23500.20495699391757413No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23430.20434648372292605No Hit
GACTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACATTTAC23190.20225330591270402No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22020.1920490640878716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20700.1805365861316504No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20230.17643744625329894No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19730.1720766591486697No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGCCAAGCCAGACCATTGGTCCTTTTTTGAATTGGTTTCAGCAGAAA19710.1719022276644845No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19480.1698962655963551No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19150.1670181461072998No Hit
GTGCAGCCACAGTTCGCTTGAATTCCACCTTGGTCCCTTGGCCGAACGTC17880.15594174686154152No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGGGTGAAGTCTGTC17690.1542846477617824No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17510.15271476440411588No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17450.15219146995156038No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17310.1509704495622642No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17250.15044715510970869No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16620.14495256335787585No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15990.139457971606043No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15480.13500996875932117No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGAAGTGACATCCAGATGACCCAGTC15400.1343122428225805No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15380.13413781133839534No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15250.13300400669119175No Hit
ATCTGGGACAGACTTCACCCTCACCATCAGCAGTCTGCAACCTGAAGATT14790.12899208255493283No Hit
GGGTGAAGTCTGTCCCAGATTCCCCGCCACTGAACCTTGATGGGACCCCA14740.1285560038444699No Hit
ACTTACTACTGTCAACAGAGTTACATTTACCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAGCGAA14590.12724776771308113No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14530.12672447326052563No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14430.12585231583959977No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGAAGTGACAT13890.1211426657666002No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13810.12044493982985953No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13770.12009607686148918No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13640.1189622722142856No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13400.11686909440406357No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGAATTCCACCTTGGTCC13350.11643301569360064No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGCCAAGCCAGA12990.11329324897826759No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA12950.11294438600989724No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12760.11128728691013813No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12410.10823473593689767No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12240.10675206832132372No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12020.10483332199528686No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11860.10343787012180551No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11740.1023912812166945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.044.7934531
TACGTAT952.3432585E-644.48170
GTGGTAT3500.042.5857771
TGGTATC3600.041.375742
CGTGCGT700.001218114340.24471370
GTATCAA33200.038.160451
GCACCGA2700.032.5948037
ATCAACG38200.032.519323
TCAACGC38250.032.4725654
TATCAAC38350.032.3963662
CAACGCA39400.031.6147965
AACGCAG40050.031.1016966
CGACCGG2850.030.87928811
CGCAGAG41750.029.6731328
AATGGTA4300.029.48159270
GTCTAGA704.3624448E-529.0357571
AGAGTAC42700.028.93368511
TGCACCG2950.028.6851276
GCAGAGT43700.028.3490459
ATAGCGA500.007152016328.04655850
Produced by FastQC (version 0.11.5)