FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763064
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGCCACAGAACTCAGCTTCAGGGACA77660.4404831588643407No Hit
GTCTATTACTGTGCGAGCGGTAATTACGATTTTTGGAGTGGTTATTCCAA63680.3611893839361475No Hit
GTATTACTGTCAGCAGTATGGTAGATCACTATTCACTTTCGGCCCTGGGA55810.31655118589001874No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40160.22778526474365085No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36010.20424669779429447No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34800.19738364574400022No Hit
GTCTCTGGTGTCTCCATCAGAACTAGTAATTACTACTGGGGCTGGATCCG33440.1896698021172232No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27850.15796363603363237No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27310.15490078635829443No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26720.15155433949079558No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26480.15019307296842316No Hit
GTGTATTACTGTCAGCAGTATGGTAGATCACTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA25100.14236579046478176No Hit
GTATGGTAGATCACTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT24110.13675056605999555No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23880.13544601897605532No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23600.13385787469995417No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23500.13329068031563235No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22890.12983079457126911No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22780.1292068807485151No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGAACTAGTAATTACTACTGGG22260.1262574699500415No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21990.12472604511237255No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21960.12455588679707601No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21670.12291102308254265No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21640.12274086476724612No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTACTTAGCCTGGTACC21380.12126615936800933No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20740.11763611530834957No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20670.11723907923932428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20130.11417622956398632No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19840.11253136584945299No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG19810.11236120753415646No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19600.11117009932708058No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19370.10986555224314035No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18950.10748333582898864No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18940.10742661639055644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18810.10668926369093805No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18700.10606534986818403No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT18620.10561159436072655No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18570.10532799716856563No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18510.10498768053797253No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18130.10283234187754954No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGCCACAGAACTCAGCTTCAGGGA17970.1019248308626346No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17960.1018681114242024No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17930.10169795310890586No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17830.10113075872458402No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17790.10090388097085529No Hit
CCACTATGGTGGGAGCACCTACTACAACCCGTCCCTCAGGAGTCGAGTCACCATATCCGTGGACACGTCCAAGAA17650.10010980883280471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)