FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763064
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGCCACAGAACTCAGCTTCAGGGACA71870.4076426040121062No Hit
GTCTATTACTGTGCGAGCGGTAATTACGATTTTTGGAGTGGTTATTCCAA62530.35466664851644636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56150.318479646796713No Hit
GTATTACTGTCAGCAGTATGGTAGATCACTATTCACTTTCGGCCCTGGGA51700.29323949669439114No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40880.2318690643107681No Hit
GTCTCTGGTGTCTCCATCAGAACTAGTAATTACTACTGGGGCTGGATCCG33760.1914848241470531No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA33540.19023699650154505No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31180.17685120903154963No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25360.14384049586401856No Hit
GTGTATTACTGTCAGCAGTATGGTAGATCACTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA24630.13969997685846913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24120.13680728549842772No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24050.13641024942940244No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23970.13595649392194498No Hit
GTATGGTAGATCACTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT23650.1341414718921151No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23150.1313054999705059No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23060.13079502502461623No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22970.13028455007872658No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22920.13000095288656566No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT22730.12892328355635418No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22220.12603059219631277No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGAACTAGTAATTACTACTGGG22010.12483948398923692No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21720.12319462027470358No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAACTACTTAGCCTGGTACC21310.12086912329898403No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20720.11752267643148517No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20140.11423294900241851No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19760.11207761034199554No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19470.1104327466274622No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19300.10946851617411506No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19040.1079938107748783No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18940.10742661639055644No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGTGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG18670.10589519155288747No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18610.10555487492229437No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT17930.10169795310890586No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17750.10067700321712655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT16700.059.4289970
GGTATCA17100.044.671971
TACGTAT1054.46131E-640.5087470
CCACGGA18500.038.047269
CGTCTGG18350.036.40365669
GTATCAA43500.035.58971
ACGTCTG17400.033.50235768
GGACGTC16950.032.81069666
TACGAAT552.5208536E-432.76479762
GACGTCT16950.032.7068567
TGTTCGC17650.032.12681656
GCTATGG18200.031.99968361
TTCGCTA17950.031.9454358
TATCAAC48350.031.656012
ATCAACG48450.031.649043
CGGTACC1109.949872E-1031.54586245
TTGTTCG18000.031.4322655
TCAACGC48450.031.156254
CGTGTCT18750.030.74966213
TCGCTAT18900.030.61386359
Produced by FastQC (version 0.11.5)