FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345002
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57680.4288469459524967No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52860.3930105680140253No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36540.2716724584796157No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32370.24066878710961026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32310.24022269111867492No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA31630.2351669365547412No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31290.23263905927277428No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA31240.23226731261366154No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29840.22185840615850388No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28690.21330823299891005No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28240.2099625130668951No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG27710.20602199848029965No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26140.19434915338415854No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25550.1899625428066278No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25290.18802946017924135No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25070.18639377487914516No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24820.1845350415835813No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24040.178735793701422No Hit
ATATATTACTGTGCGAAAGATCCCGGGCGGGAAGACCCAAAAGGGAGCCGCGAGGGCACCCTGGTCACCGTCTC23850.17732315639679344No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23650.17583616976034236No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC23560.17516702577393936No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTCTTAGTATCTGGTTGGCCTGGTATCAGCAGAAA22440.16683990060981324No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21550.16022281007760583No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21290.1582897274502194No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21050.1565053434864781No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20450.1520443835771248No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA20410.15174698624983457No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT19920.14810386899052938No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18960.14096633313556411No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18780.13962804516275812No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18550.13791801053083935No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18500.13754626387172658No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18470.13732321587625893No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17840.13263920797143797No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17530.13033437868493875No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17370.12914478937577786No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGATACTA16860.1253529734528276No Hit
GGACTGAACAGAGAGAACTCACCATGGACTTTGGGCTGAGCTGGCTCTTT16640.12371728815273138No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTCTTAGTAT16570.12319684282997348No Hit
GTCTTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT16540.12297379483450581No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16310.12126376020258707No Hit
ACTTATTACTGCCAACACTATGATAATTATTCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA16290.12111506153894196No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16010.1190332802479104No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATGATAATTATTCCTGGACGTT15470.11501841632949245No Hit
GTCAGAGTCTTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCTTGATCTATAAGA15310.11382882702033158No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15280.1136057790248639No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15220.11315968303392858No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15160.11271358704299324No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCTTCCACC14960.11122660040654214No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14850.11040875775649404No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG14810.11011136042920382No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC14730.10951656577462339No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCGCCTATGCCATGACCTGGATCCGCCAGGCTCCAGGGAAGGGG14640.10884742178822039No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCC14550.10817827780181738No Hit
GAGTCTTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCTTGATCTATAAGACAT14540.10810392846999484No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14380.10691433916083395No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14330.1065425925017212No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14320.10646824316989864No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14200.10557605118802797No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG14060.1045351605425122No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14010.10416341388339942No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC13920.10349426989699645No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT13880.10319687256970622No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13770.10237902991965811No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13720.10200728326054534No Hit
CTATAAGACATCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCT13680.10170988593325513No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13600.10111509127867467No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13590.10104074194685211No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13570.100892043283207No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13570.100892043283207No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13550.10074334461956189No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13530.1005946459559168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT353.3094875E-759.08599539
ACCGATC2200.044.89843827
TATCGCG704.638423E-1044.15960336
GACCGAT2250.043.8663626
ATCGCGT651.1781594E-842.29787437
CTTATAC2750.042.0176731
GTATCGC759.1858965E-1041.17848635
GAGACCG2400.041.10022424
ACGAGAC2500.040.80158622
TCTGGAG14700.040.4625970
AGACCGA2450.040.26893625
CCGATCA2550.040.08806628
TCTCCGA2850.039.3362911
CCCACGA2850.036.9701419
AGTATCG1300.036.90239334
CGATCAG2800.036.5155829
GTATCAA34000.036.3838161
ATCTCCG3000.036.2370710
CGAGCCC3100.036.16938815
CACGAGA2800.035.21172321
Produced by FastQC (version 0.11.5)