FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345002
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55280.4110031063150835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46620.34661658495675096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40800.30334527383602405No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA30610.22758330470884058No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29970.22282494747219708No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA29830.2217840568266813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29540.2196279262038272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28630.2128621370079747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27920.2075833344485733No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26940.20029709992996295No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26570.19754617465252838No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25890.19249042008859465No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25860.192267372093127No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25800.19182127610219168No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG24220.180074081674228No Hit
ATATATTACTGTGCGAAAGATCCCGGGCGGGAAGACCCAAAAGGGAGCCGCGAGGGCACCCTGGTCACCGTCTC23690.1761335670876326No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22960.1707060658645861No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22750.16914472989631243No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22690.1686986339053771No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22370.16631945528705533No Hit
GTGCAGCCACAGTTCGTTTCATTTCCACCTTGGTCCCTTGGCCGAACGTC22150.16468376998695913No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21890.16275068735957268No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21810.16215589270499225No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20000.14869866364510984No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19910.1480295196587068No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19840.14750907433594895No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTCTTAGTATCTGGTTGGCCTGGTATCAGCAGAAA19840.14750907433594895No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA18730.13925629850364535No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18610.1383641065217747No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18270.13583622923980784No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18210.1353901332488725No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18050.13420054393971162No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT18030.1340518452760665No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16860.1253529734528276No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA16630.12364293882090882No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15730.11695149895687887No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGATACTA15710.11680280029323377No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTCTTAGTAT15680.11657975229776611No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT15620.11613365630683077No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15570.11576190964771799No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15550.11561321098407287No Hit
GTCTTAGTATCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT15420.11464666967037968No Hit
ACTTATTACTGCCAACACTATGATAATTATTCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATGAAACGAA15330.11397752568397666No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15230.11323403236575114No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACCGCCTATGCCATGACCTGGATCCGCCAGGCTCCAGGGAAGGGG15220.11315968303392858No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15210.11308533370210602No Hit
GGACTGAACAGAGAGAACTCACCATGGACTTTGGGCTGAGCTGGCTCTTT15140.11256488837934812No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACACTATGATAATTATTCCTGGACGTT14970.1113009497383647No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14970.1113009497383647No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14920.11092920307925194No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14640.10884742178822039No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14410.10713738715630163No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14060.1045351605425122No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13930.103568619228819No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13780.10245337925148065No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCTTCCACC13760.10230468058783555No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13550.10074334461956189No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13530.1005946459559168No Hit
GTCTTATAGATCAAGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGATACTAAGACTCTGA13490.10029724862862657No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13460.10007420063315892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT758.54925E-1173.6612870
TCTGGAG11750.045.84239270
GTACGTA902.1485903E-839.5114269
GTATCAA35750.038.528731
GTGGTAT3800.034.8189541
TACGACT400.00282882333.902194
TCAACGC41250.033.1214144
ATCAACG41200.032.998263
CAACGCA41650.032.723145
TATCAAC41450.032.722312
AACGCAG42150.032.498256
TGGTACG951.6398735E-731.36063867
AATGGTA5300.031.27129670
CGCAGAG45550.030.0724758
GGTACGT1052.420893E-729.99734368
AGAGTAC46000.029.92570511
CAGAGTA46050.029.81958410
GCAGAGT47100.029.1548189
CGACCGG4700.028.85614611
GAATTGT11600.028.48179862
Produced by FastQC (version 0.11.5)