FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739867
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42590.2447888258125477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39710.22823583641738135No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33240.191049085935879No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32360.1859912280651337No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27380.15736835056932513No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27360.1572533992540809No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGACACAGATTTCA26730.15363243282388828No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAA25880.1487470019260093No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25180.1447237058924619No Hit
GGATATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATGCTGCATC24870.14294196050617664No Hit
CTACTATTGTCAACAGGCTCACAGTTTCCCGCTCACTTTCGGCGGAGGGA23880.13725187040158818No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23520.13518274672719238No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22570.12972255925309234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22330.1283431434701618No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTCCGCCGAAAGTG21730.12489460401283545No Hit
GGCATACACTGGGTCCGTCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAATGATATCATATGATGGAAGTAAT21310.12248062639270703No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20590.11834237904391542No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20580.11828490338629333No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20320.11679053628811857No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20140.11575597445092067No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19750.11351442380365855No Hit
ATAATACACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATACA19710.11328452117317013No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19570.11247986196646065No Hit
ATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGACACAGATTTCACTCTCA19370.11133034881401853No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTCCGCCGAAAGTGAGCGGGAAA18990.10914627382437854No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18980.10908879816675643No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18890.10857151724815747No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18800.10805423632955853No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18590.1068472475194943No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18530.10650239357376168No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18090.10397346463838902No Hit
TCAGTAGCCTGCAGCCTGAAGATTTTGCAATCTACTATTGTCAACAGGCT18030.10362861069265639No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18010.10351365937741218No Hit
CAATAATACACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATA17980.10334123240454587No Hit
CAATAGTAGATTGCAAAATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTGTCAGATCCACTGCCG17690.1016744383335048No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17440.10023754689295215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG651.2529199E-842.00533734
GCGAATC2500.038.18280433
GTATCAA51650.037.098071
GGCGAAT3450.036.52097332
TAAGGCG3900.035.69334829
GACTAAG4150.034.30291726
GGTACGG601.5286092E-533.8536261
ACGAGAC4150.033.44574722
TATCAAC57700.032.7350962
ACTAAGG4250.032.71333727
ATCAACG59050.031.9293843
CAACGCA60000.031.5366675
TCAACGC59950.031.5065084
CTTATAC4750.031.3591461
CGAGACT4450.031.19456723
AACGCAG60600.031.1685666
AATCGCG553.424943E-431.0969736
AATGGTA8550.030.45458270
AGACTAA4450.030.4503625
CACGAGA4450.030.427621
Produced by FastQC (version 0.11.5)