FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739867
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45740.262893657963511No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40420.23231660810855084No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33360.19173879382734427No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33030.1898420971258148No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32310.18570384977702317No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25940.14909185587174192No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGACACAGATTTCA25540.14679282956685769No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24480.1407004098589145No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23530.13524022238481445No Hit
GGATATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAAGCTCCTGATCTATGCTGCATC23160.13311362305279656No Hit
CTACTATTGTCAACAGGCTCACAGTTTCCCGCTCACTTTCGGCGGAGGGA22600.12989498622595863No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22510.1293777053073597No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22430.12891790004638284No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAGCAGGTGGTTAGCCTGGTATCAGCAGAAA22000.12644644676863231No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTCCGCCGAAAGTG21660.12449227440948074No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21330.12259557770795124No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20580.11828490338629333No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20490.11776762246769436No Hit
GGCATACACTGGGTCCGTCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGCAATGATATCATATGATGGAAGTAAT20160.1158709257661649No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19280.1108130678954196No Hit
ATAATACACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATACA19200.11035326263444274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18980.10908879816675643No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18650.10719210146522694No Hit
CAATAGTAGATTGCAAAATCTTCAGGCTGCAGGCTACTGATGGTGAGAGTGAAATCTGTGTCAGATCCACTGCCG18540.10655986923138379No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18480.10621501528565115No Hit
GTGTATTATTGTGCGAAACCGATTCCTAATAATTACTATCTTGACTATTGGGGCCACGGAAGCCTGGTCACCGT17870.10270900017070271No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTCCGCCGAAAGTGAGCGGGAAA17740.10196181662161534No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17720.10184686530637112No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17700.1017319139911269No Hit
ATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCGAGGTTCAGCGGCAGTGGATCTGACACAGATTTCACTCTCA17690.1016744383335048No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17660.1015020113606385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)