FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1589178
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGTTCACCTCTCACTTTCGGCGGAGGGA61530.3871812975009722No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47080.2962537865487692No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA43150.27152402059429465No Hit
GTGTTAGGAGCACCTACTTAGCCTGGTACCAGCAGAGACCTGGCCGGGCTCCCAGGCTCCTCATCTATGGTTCAT43060.27095769007625325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38190.24031291648890182No Hit
GTATGGTAGTTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT37110.23351695027240496No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA31200.19632791292101956No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC30820.19393673962262253No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30510.19198604561603544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29340.18462374888149724No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA26800.16864064315010652No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26320.16562021372055238No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTG26300.16549436249432098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24860.15643307420565852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24640.15504871071711288No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT23080.1452323150710619No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22300.14032411724803642No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21890.13774416711029225No Hit
CAATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTGAGGTTCAGGGAGAACTGGTTCTTGGACCTGTCTA21630.13610810116928374No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21570.1357305474905895No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21210.13346522541842387No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21010.13220671315610963No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20930.13170330825118393No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20880.13138868018560537No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTGCCAGACCCACTGCCA20740.13050772160198543No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20170.12692096165438987No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC19170.12062840034281874No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19070.11999914421166162No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18870.1187406319493474No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18620.11716749162145462No Hit
ATAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTGAGGTTCAGGGAGA18230.11471339270994188No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC18150.11420998780501618No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18070.11370658290009049No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17860.11238514502466056No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGTTCA17800.11200759134596629No Hit
GTATTATTGTGCGAGATGGGCGGGGGGAGTGCCAACTGAAGATCGGTACTACTTTGACTACTGGGGCCAGGGAAT17800.11200759134596629No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCTGGGCCAGTCACAGTGTTAGGAG17780.11188174011973485No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17730.1115671120541563No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17280.10873545946394929No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT17190.10816912894590787No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG16670.1048969970638909No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16660.10483407145077518No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16620.10458236899831233No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16130.10149901395564247No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16100.10131023711629533No Hit
ATCCAGGAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGCACAGACTTCACTCTCACCATCAG15980.1005551297589068No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15960.10042927853267539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTG200.00758039651.3644228
GGTATCA15700.047.052011
TCGTATG751.6370905E-1146.03151740
CGTATGC803.45608E-1143.19709441
ATCTCCG2350.042.19077710
CGAGACG2200.040.4243523
ACGAGAC2450.039.0867622
CGTAGAG2300.038.7094228
GTATCAA43550.037.930021
ACGTAGA2350.037.87388227
TACGCGG400.002439786734.94104452
GAGACGT2550.034.87920824
CACGAGA2750.034.8194621
CCCACGA2750.034.81727219
ATCTCGT905.296897E-934.42956537
AATCTCG1003.929017E-1034.40558236
TATCAAC48400.033.9139942
AGACGTA2650.033.5661825
ATCAACG49250.033.4675453
CAACGTT652.2841939E-433.31886769
Produced by FastQC (version 0.11.5)