FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526811
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAATGTCCCCATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT74340.48689719945690724No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66650.4365307821334795No Hit
TTATCATACTGTTGACAATAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATCTGTC61350.40181790673501827No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50030.3276764445632105No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49860.32656301271080707No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48700.31896547771793626No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG48130.3152322062128188No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39290.2573337498878381No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCACAACTATTTAAATTGGTATCAGCAGAAA37620.24639591933775695No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36010.23585106473558284No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34740.22753307383821575No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34660.22700910590767293No Hit
GAGTAAAATCTGTCCCAGATCCACTTCCACTGAACCTTGATGGGACCCCT33410.2188221069929415No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31710.20768778846890676No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTCACAA30910.20244810916347863No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30860.2021206292068894No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30220.19792888576254689No Hit
ATATTATTGTCAACAGTATGATAATGTCCCCATCACTTTCGGCCAAGGGA29910.1958985100316935No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29810.195243550118515No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29050.19026585477835828No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28600.18731853516905497No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28550.18699105521246573No Hit
GATTTTACTCTCACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTATTGTCAACAGTATGATAATGTC28270.18515716745556587No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28050.18371625564657315No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27360.1791970322456414No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27330.17900054427168785No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT26540.1738263609575776No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT23860.1562734352843934No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23860.1562734352843934No Hit
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGAGACA23670.15502901144935424No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23520.15404657157958646No Hit
AGTCAGGACATTCACAACTATTTAAATTGGTATCAGCAGAAACCAGGGAA23150.15162321990082597No Hit
CAATAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTAAAATCTGTCCCAGATCCACTTCCA22850.14965834016129043No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22580.14788994839570843No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC22540.14762796443043702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22120.1448771327950873No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC21280.13937546952438776No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT21110.13826203767198428No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG20700.13557670202795238No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20690.13551120603663452No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAAGTG20550.1345942621581846No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20460.13400479823632394No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT19920.13046801470515998No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19720.12915809487880292No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19470.12752069509585665No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18750.12280498372097136No Hit
GTGTATTACTGTGTGAGAAAGTATGATACTAGTGCATCTTACTACGGCCG18700.1224775037643821No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18670.12228101579042855No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA17190.11258760907538654No Hit
CAGTATGATAATGTCCCCATCACTTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT16880.11055723334453314No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16840.11029524937926174No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16700.10937830550081182No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16690.10931280950949399No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16660.1091163215355404No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16470.10787189770050125No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16370.10721693778732273No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16340.10702044981336918No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGAGA16300.10675846584809777No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16000.10479358610856224No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC15980.10466259412592652No Hit
GTCGTGTCCCTTGGCCGAAAGTGATGGGGACATTATCATACTGTTGACAATAATATGTTGCAATATCTTCAGG15940.10440061016065512No Hit
ACCTTGATGGGACCCCTGTTTGCAAATTGGATACATCGTGGATCAGGAACTTAGGGGCTTTCCCTGGTTTCTG15800.10348366628220519No Hit
ATCATACTGTTGACAATAATATGTTGCAATATCTTCAGGCTGCAGGCTGC15800.10348366628220519No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCACCTGAGAGCCAGAG15790.10341817029088733No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15690.10276321037770884No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG15670.10263221839507312No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15670.10263221839507312No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15530.1017152745166232No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTATTGTCAACAGTATGATAATGTCCCCATCACTTT15420.10099481861212685No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT15380.10073283464685544No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15350.1005363466729019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG252.7801996E-454.317442
GTACGTC252.7815497E-454.3121033
GTGGTAT3300.041.173861
GTATCAA37400.039.4179151
TATCAAC40200.036.059622
TCAACGC41250.035.050254
ATCAACG41500.034.844813
CAACGCA42250.034.2206575
CGGGTAT701.0996155E-633.96843317
CTAACGC502.0477924E-433.9450653
AACGCAG42800.033.85916
TCTGGAG11900.033.70005870
GTTATTA1302.0312462E-532.4721270
CGCAGAG45700.031.7104918
ATACGTA2250.031.67583511
GTGCTAG2500.031.2509571
AGAGTAC47150.030.735311
GCAGAGT47700.030.309769
CAGAGTA47900.030.18320510
GAATACG2400.029.6960939
Produced by FastQC (version 0.11.5)