FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262100
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47270.37453450598209337No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40700.3224784090008716No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37900.3002931621900008No Hit
AACCAGGACAGCCTCCTAAACTTCTCACATACTGGGCTTCTACCCGGGCA37050.293558355122415No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31760.2516440852547342No Hit
ATCTTGGTTTTCCTCGCACTTTCGGCGGAGGGACCAGACTTGAGATCAAA29550.23413358687901117No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28900.2289834402979162No Hit
GTTTATTACTGTCAGCACTATCTTGGTTTTCCTCGCACTTTCGGCGGAGGGACCAGACTTGAGATCAAACGAA28510.22589335234925917No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28490.2257348863006101No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCA28430.22525948815466287No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27980.22169400206005865No Hit
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGTGACTG27640.2190000792330243No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26370.20893748514380794No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25210.19974645432216148No Hit
CTCCTAAACTTCTCACATACTGGGCTTCTACCCGGGCATCCGGGGTCCCT25160.1993502892005388No Hit
GTGCAGCCACAGTTCGTTTGATCTCAAGTCTGGTCCCTCCGCCGAAAGTG24850.19689406544647808No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23980.19000079233024325No Hit
GTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAATTCTTATTGTTGTGACTGGAGAAAAGACT23220.18397908248157832No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCAAGTCTGGTCC22900.18144362570319308No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21720.17209412883289754No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21690.17185642975992393No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21380.16940020600586322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21020.16654781713017985No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20660.16369542825449648No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20440.16195230171935662No Hit
GTACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC20390.16155613659773393No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC20340.16115997147611125No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19930.15791141747880516No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19480.15434593138420094No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19350.15331590206798193No Hit
GTCCCTGACCGATTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCAC19250.15252357182473655No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG19140.15165200855716662No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATCTTGGTTTT18600.14737342524364155No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18520.14673956104904526No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17660.13992552095713492No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16890.13382457808414547No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTCTC16840.13342841296252278No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16650.13192298550035655No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTCTCCAGTCACAA16290.12907059662467316No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16250.12875366452737502No Hit
GAGTGAAATCTGTCCCAGACTCGCTGCCACTGAATCGGTCAGGGACCCCG16230.12859519847872594No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16220.1285159654544014No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15840.12550511053006894No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15350.12162269233816655No Hit
AACCAAGATAGTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT15350.12162269233816655No Hit
GTCCCAGACTCGCTGCCACTGAATCGGTCAGGGACCCCGGATGCCCGGGT15060.11932493463275493No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA14970.11861183741383409No Hit
ATTCAGTGGCAGCGAGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC14970.11861183741383409No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14880.11789874019491324No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14860.11774027414626416No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14680.11631407970842247No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATCTTGGTTTTCCTC14640.11599714761112431No Hit
GTACCAGCACAAACCAGGACAGCCTCCTAAACTTCTCACATACTGGGCTTCTACCCGGGCATCCGGGGTCCCTGA14640.11599714761112431No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCACTGAATCGGTCAGGGACC14420.11425402107598448No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14390.11401632200301086No Hit
GTCAGGGACCCCGGATGCCCGGGTAGAAGCCCAGTATGTGAGAAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTA14380.11393708897868632No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14330.11354092385706363No Hit
GTATGTGAGAAGTTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTA14280.11314475873544093No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACTCGCTGCCA14240.11282782663814277No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14220.11266936058949371No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13420.10633071864353064No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATCTTGGTTTTCCTCGCACTTT13270.10514222327866254No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13090.10371602884082086No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12850.10181443625703193No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12840.10173520323270739No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC12830.10165597020838285No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12750.10102210601378656No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12670.10038824181919023No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12650.10022977577054115No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12630.1000713097218921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)