FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997371
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATGACTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT37140.3723789843498558No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26020.26088586894946814No Hit
GTCATTATACTGATGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT24940.2500574009069845No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA19090.1914031990101978No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18950.18999950870839435No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17920.1796723586308405No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17730.17776735036410724No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17080.17125021682001984No Hit
GTATTACTGTGCGAGAGAAAAGGGACCGTATTATTATGATGGTGGTCATT15780.15821594973184502No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15710.1575141045809433No Hit
GAGTATTAGTAGCAGCTTAGCCTGGTACCAACAGAGACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATC14890.14929248995609457No Hit
CAGTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATG14690.14728721809637535No Hit
CATCTATGGTGCATCCACCAGGGCCCCTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA14440.14478062827172636No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCATCAGTATAATGACTGG14260.1429758835979791No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13800.13836375832062492No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13160.13194688836952348No Hit
GTAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATGCA12860.12893898057994466No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12820.12853792620800084No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12630.1266329179412676No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGTGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTATTAGTAG12480.1251289640464782No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGGGGCCCTGGT12250.12282290140780111No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11890.11921341206030654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11760.11790998535148906No Hit
GTACCAACAGAGACCTGGGCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCCCTGGTATCCCAGC11310.11339812366712085No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11260.11289680570219106No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11110.11139285180740166No Hit
CTGTATTACTGTGCGAGAGAAAAGGGACCGTATTATTATGATGGTGGTCATTATTATCCATACTACTTTGACCA10960.10988889791261225No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTCTCTGTTGGTACCAGGC10920.10948784354066841No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10840.10868573479678074No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10720.10748257168094921No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGTAGCAGCTTAGCCTGGTACCAACAGAGACCTGGGCAGGCTCCCAGGCT10580.10607888137914578No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC10420.10447466389137042No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10360.10387308233345464No Hit
GTTTATTACTGTCATCAGTATAATGACTGGCCTCCGATCACCTTCGGCCA10140.10166728328776353No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCGA306.479299E-445.75499737
GGTATCA9700.042.7472761
TACACGC451.0263885E-438.17361538
ATCTCGT1550.037.63717737
CGTATGC1850.037.31780641
TCGTATG2000.034.44879540
GTATCAA24850.034.0559771
CGAGTTC956.150003E-933.9039160
ATGCCGT2150.032.23049544
ACGAGAC2450.031.9179222
TTGCCGA1001.12486305E-831.86882656
CCCACGA2600.031.37473519
AATACGA450.00417785131.30692753
CACGAGA2650.030.79202321
CAGTTCG11850.030.70963370
TCTCCGA2600.030.05991711
GTGGTAT2500.029.9090791
CCACTGG7900.029.8565970
AATGGTA7500.029.65185570
ATCAACG29000.029.5325623
Produced by FastQC (version 0.11.5)