FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911409
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39620.4347115290720192No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39120.42922551785202917No Hit
GAGTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT31620.3469353495521769No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25530.2801157328926969No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25270.27726300705830204No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG24410.267827067759919No Hit
GAATTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGT23960.26288965766192784No Hit
GTAGGAGACAGAGTCACCATCAGTTGCCGGACAAGTCAGAACATTAACATCTTTTTAAATTGGTATCAGCAAAAA22440.24621218355315783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22260.24423721951396135No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22190.24346917794316275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21820.23940952964037004No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21380.2345818397667787No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGGTGGTCATTTGCAGATATACTAAGTTCTTGACATTGTCTCTGGA20390.22371953755119822No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATT20340.22317093642919916No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19880.21812380610680823No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19520.21417387802841534No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19510.21406415780401553No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19030.20879758703282503No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAATTCTGTC18550.20353101626163447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18380.20166577244683778No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17970.1971672432464459No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17960.19705752302204607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGGGCGGAGACTA17930.1967283623488467No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17750.19475339830965022No Hit
ACTTACTACTGTCAACAGAGTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA17530.19233955337285455No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGGG17230.18904794664086047No Hit
AGTCAGAACATTAACATCTTTTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTTTG16940.18586606013326618No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16710.18334249497207072No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16280.1786245253228792No Hit
GTCCCAGACCCACTGCCATTGAATCTTGATGGGACCCCACTTTGCAAACT15610.1712732702880924No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15510.17017606804409438No Hit
GGCCAAAAGGGCGGAGACTACTGTAACTCTGTTGACAGTAGTAAGTTGCA15270.1675427826584991No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15120.16589697929250205No Hit
CTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAATT14980.16436089615090482No Hit
GTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGA14820.16260537256050797No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14780.16216649166290875No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGA14690.16117900964331053No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCATTGAATCTTGATGGGACCCCA14660.16084984897011112No Hit
GTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGACCAAGTTGGAGATCAAA14250.1563513197697192No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14170.15547355797452078No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14160.15536383775012097No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14050.15415691528172312No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAA13770.15108474899852867No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13500.14812230293973397No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13100.14373349396374185No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA13070.14340433329054245No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13060.14329461306614263No Hit
GTCTATTACTGTGCGAGAGAGCCCGCCGGGGGGTATAACTGGAACACCCT12300.13495587601175763No Hit
GGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAAAGATGTTA12050.13221287040176255No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTTC11600.12727546030377143No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11510.12628797828417318No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11410.12519077604017514No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11320.12420329402057692No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11300.12398385357177731No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11260.12354497267417812No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11040.12113112773738245No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11010.12080196706418303No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10930.1199242052689846No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT10760.11805896145418797No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10720.11762008055658875No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10720.11762008055658875No Hit
GAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCC10600.11630343786379113No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10550.1157548367417921No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10530.1155353962929925No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10440.11454791427339428No Hit
GCCCCTAACCTCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAATGGCAGTGGGT10300.11301183113179704No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10220.11213406933659861No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10140.11125630754140019No Hit
ACAGTAGTCTCCGCCCTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGA10100.11081742664380097No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10060.11037854574620176No Hit
GTGGGGTCCCATCAAGATTCAATGGCAGTGGGTCTGGGACAGAATTCACT9780.10730637946300729No Hit
CCATCGTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCG9590.10522169519941102No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGT9580.10511197497501121No Hit
GTCTCCATCGTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCGGACAAGTCAGAACATTAACAT9550.10478281430181181No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9520.1044536536286124No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC9490.10412449295541298No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9450.10368561205781378No Hit
CCCCTAACCTCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCA9410.10324673116021456No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9370.10280785026261535No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9240.10138148734541792No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9220.10116204689661831No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9190.10083288622341889No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9190.10083288622341889No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9150.1003940053258197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTG200.00764682651.2494248
TGAGCGA2200.046.76226831
ACGAGAC2500.046.4916522
TCGTATG1050.045.9788140
CGTATGC1150.045.02414741
GAGCGAT1850.044.51921532
ATCTCCG2600.043.364910
CACGAGA2700.043.04073321
CGAGCCC2850.040.75977315
CGAGACA3050.040.35619423
TCTCCGA2800.040.26740311
CTTATAC2850.039.5783271
CCCACGA3000.038.7302919
AGCGATG1350.038.148833
GTCGTAT557.452749E-637.5919739
AGACACT3100.037.51797525
GAGACAC3100.037.5035624
TTATACA3250.036.8025362
TCCGAGC3100.036.3705613
GTATCAA22800.036.1302151
Produced by FastQC (version 0.11.5)