FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911409
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41380.4540222885663846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33460.36712387084174064No Hit
GAGTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCAT31920.350226956284171No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26120.28658922613228527No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25130.2757269239167048No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23490.25773280711513713No Hit
GTAGGAGACAGAGTCACCATCAGTTGCCGGACAAGTCAGAACATTAACATCTTTTTAAATTGGTATCAGCAAAAA22930.25158847454874816No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22620.24818714759235425No Hit
GAATTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGT21640.23743456560117357No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21470.2355693217863769No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21360.2343623993179791No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20330.22306121620479938No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATT20000.2194404487996059No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGGTGGTCATTTGCAGATATACTAAGTTCTTGACATTGTCTCTGGA19580.21483219937481415No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19540.21439331847721496No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19000.2084684263596256No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18160.19925192751004214No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAATTCTGTC17870.19607004100244785No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17840.19574088032924844No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17400.19091319045565713No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17320.1900354286604587No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGGG17110.18773130394806284No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16940.18586606013326618No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAACTTGGTCCCCTGGCCAAAAGGGCGGAGACTA16520.18125781070847446No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16400.17994116801567683No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16360.1795022871180776No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15930.1747843174688861No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15790.17324823432728884No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15700.1722607523076906No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15490.16995662759529476No Hit
GTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGA15460.16962746692209535No Hit
ACTTACTACTGTCAACAGAGTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAA15140.16611641974130167No Hit
AGTCAGAACATTAACATCTTTTTAAATTGGTATCAGCAAAAACCAGGGAAAGCCCCTAACCTCCTGATCTTTG15000.16458033659970442No Hit
GGCCAAAAGGGCGGAGACTACTGTAACTCTGTTGACAGTAGTAAGTTGCA14740.16172761076530953No Hit
GTCCCAGACCCACTGCCATTGAATCTTGATGGGACCCCACTTTGCAAACT14740.16172761076530953No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14420.15821656358451583No Hit
GAGTGAATTCTGTCCCAGACCCACTGCCATTGAATCTTGATGGGACCCCA14030.15393747483292353No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13970.1532791534865247No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGA13890.15240139169132627No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13720.15053614787652964No Hit
CTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAATT13700.15031670742773004No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13390.14691538047133615No Hit
GTTACAGTAGTCTCCGCCCTTTTGGCCAGGGGACCAAGTTGGAGATCAAA13250.1453792973297389No Hit
GTGGGGTCCCATCAAGATTCAATGGCAGTGGGTCTGGGACAGAATTCACT13170.14450153553454048No Hit
CTTTTGGCCAGGGGACCAAGTTGGAGATCAAACGAACTGTGGCTGCACCA12730.13967384566094915No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12730.13967384566094915No Hit
GTCTATTACTGTGCGAGAGAGCCCGCCGGGGGGTATAACTGGAACACCCT12710.13945440521214955No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAA12630.1385766434169511No Hit
GGTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAATTTAAAAAGATGTTA12260.1345169951141584No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12200.1338586737677596No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCGTTC12130.133090632196961No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11970.13133510860656414No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11950.13111566815776451No Hit
GCCCCTAACCTCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGATTCAATGGCAGTGGGT11820.1296893052405671No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11780.12925042434296785No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA11760.12903098389416826No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11350.12453245469377634No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11320.12420329402057692No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11290.12387413334737753No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10890.11948532437138541No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10860.11915616369818599No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10850.11904644347378618No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10830.1188270030249866No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10750.11794924122978817No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10570.11597427719059171No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10500.11520623561979308No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10080.11059798619500137No Hit
GTCTCCATCGTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCAGTTGCCGGACAAGTCAGAACATTAACAT10050.11026882552180195No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGAGTTTGTGTTT9750.10697721878980787No Hit
CCCCTAACCTCCTGATCTTTGCTGCATCCAGTTTGCAAAGTGGGGTCCCA9590.10522169519941102No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9480.1040147727310132No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9460.10379533228221359No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9300.10203980869181674No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT9210.1010523266722185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)