FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004692365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341047
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29310.21856057244824378No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26610.19842704990951102No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC26190.19529516862570812No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25330.18888226885411175No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24410.18202195747054353No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24370.1817236830625623No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT23560.17568362630094247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21950.1636780813796981No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21840.16285782675774974No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT20480.15271649688638803No Hit
ACAGTATTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGACATCAAGCGA20070.14965918420458044No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA18750.13981612874119997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18180.13556571842746748No Hit
GAGTTACAGTATTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGACATCAAGCGAACTGTGGCTGCACCAT17600.13124073951173973No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17200.12825799543192745No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17080.12736317220798377No Hit
GAATACTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAATGAAAT17010.1268411919940166No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16250.1211739782423733No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16140.12035372362042493No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG15900.11856407717253759No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15650.11669986212265492No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15500.11558133309272532No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14160.10558914042535421No Hit
CTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAATGAAATCTGTC14080.10499259160939177No Hit
GTGTAAATTGGATGCAGCAGATATCAGGACCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGT13790.10283010215152788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)