FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004692366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004692366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341047
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27230.20305030323322001No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC26150.1949968942177269No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24940.1859740933762948No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24780.1847809957443699No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT24570.18321505510246844No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23790.17739870414683453No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22520.1679284916934306No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21220.15823457343404074No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20600.1536113201103317No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT20150.1502557330205429No Hit
GAGTTACAGTATTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGACATCAAGCGAACTGTGGCTGCACCAT18990.14160577518908735No Hit
ACAGTATTCCGTTCACTTTTGGCCAGGGGACCAAGCTGGACATCAAGCGA18440.13750450207934548No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA17900.1334777975715989No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17680.13183728832770217No Hit
GAATACTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAATGAAAT17320.12915281865587114No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17260.1287054070438993No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17020.12691576059601192No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16770.12505154554612927No Hit
GTGTAAATTGGATGCAGCAGATATCAGGACCCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGGT16030.11953346899847657No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG15810.11789295975457982No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15150.11297143202288956No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14990.11177833439096467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14500.10812447289319463No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCGGTGGATCTGGGACAGATTTCATT14350.10700594386326505No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14000.1043960427934293No Hit
CTGTAACTCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAATGAAATCTGTC13940.10394863118145746No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT2850.044.2223170
GCGGAAC400.001265354539.93812668
CCTAACG451.16076524E-437.3852962
GTATCAA64650.034.8268621
TATCAAC65050.033.9829752
GTACGGC400.002940484333.63547514
ATCAACG67050.033.01583
TCAACGC67250.032.8688134
CAACGCA69250.032.01675
GGTATCA31500.031.732381
AACGCAG70950.031.2495636
GTAACGG450.00481455730.4130837
GTGGTAT6000.030.291
ATAGCGG1101.9417712E-830.13237263
ACGCAGA74350.029.820537
CGCAGAG74950.029.5818068
GTACGTA3250.028.96349569
GGTACGT2900.028.9207168
AGAGTAC76550.028.87560711
GCAGAGT77750.028.5164839
Produced by FastQC (version 0.11.5)